COMPARISON OF MYCOBACTERIA GROWTH INDICATOR TUBE WITH BACTEC-460TB SYSTEM AND LOWENSTEIN-JENSEN MEDIA FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS

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MOHAMMADI F.; FARNIYA P.; GHAZI SAEIDI KIOUMARS; BAHADORI K.; MASJEDI M.R.; VELAYATI A.A.

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Title

COMPARISON OF MYCOBACTERIA GROWTH INDICATOR TUBE WITH BACTEC-460TB SYSTEM AND LOWENSTEIN-JENSEN MEDIA FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS

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MGITQ4

BACTECQ4

LOWENSTEIN-JENSENQ4

Abstract

The mycobacterium growth Indicator tube (MGIT) was evaluated and compared with the BACTEC-460 TB system and Lowenstein - Jensen (LJ) medium for isolation capactiy and detection time of mycobacteria.Sputum specimens were collected from 156 patients admitted to NRITLD (Aug 98-Mar.99) with clinical signs of pulmonary tuberculosis. Specimens were decontaminated using the standard NaOH- NALC method, then processed in MGIT broth, in BACTEC vial and on LJ media for comparative study. Smears from sample sediment of all specimens were prepared and stained with Ziehl Neelsen.the result of our study shows that:1) The recovery rate in smear positive cases (n=82) with LJ medium was 90% with MGIT was 87.8%, and with BACTEC-460 was 89%.2) The recovery rate in smear negative cases (n=74, with clinical sign of TB) with LJ medium was 94%, 86% with MGIT and it was 94% with BACTEC-4603) The mean time of detection was 6.6 day (range: 2-12) in MGIT,11.7 days (range:10-15) in BACTEC, 27 days (range: 25-42) in LJ medium4) Overall, the sensitivity of LJ was 92%, MGIT 88% and BACTEC 92.2% whereas the specificity of them was 93%, 88% and 90% respectively.The MGIT is an easy, rapid culture media and can be used in addition to L.J culture media for clinical specimens.

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APA: Copy

MOHAMMADI, F., FARNIYA, P., GHAZI SAEIDI, KIOUMARS, BAHADORI, K., MASJEDI, M.R., & VELAYATI, A.A.. (2002). COMPARISON OF MYCOBACTERIA GROWTH INDICATOR TUBE WITH BACTEC-460TB SYSTEM AND LOWENSTEIN-JENSEN MEDIA FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS. JOURNAL OF MEDICAL COUNCIL OF I.R.I., 19(4), 293-298. SID. https://sid.ir/paper/41454/en

Vancouver: Copy

MOHAMMADI F., FARNIYA P., GHAZI SAEIDI KIOUMARS, BAHADORI K., MASJEDI M.R., VELAYATI A.A.. COMPARISON OF MYCOBACTERIA GROWTH INDICATOR TUBE WITH BACTEC-460TB SYSTEM AND LOWENSTEIN-JENSEN MEDIA FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS. JOURNAL OF MEDICAL COUNCIL OF I.R.I.[Internet]. 2002;19(4):293-298. Available from: https://sid.ir/paper/41454/en

IEEE: Copy

F. MOHAMMADI, P. FARNIYA, KIOUMARS GHAZI SAEIDI, K. BAHADORI, M.R. MASJEDI, and A.A. VELAYATI, “COMPARISON OF MYCOBACTERIA GROWTH INDICATOR TUBE WITH BACTEC-460TB SYSTEM AND LOWENSTEIN-JENSEN MEDIA FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS,” JOURNAL OF MEDICAL COUNCIL OF I.R.I., vol. 19, no. 4, pp. 293–298, 2002, [Online]. Available: https://sid.ir/paper/41454/en

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