INVESTIGATION OF ANTIBACTERIAL PROPERTIES OF ETHANOLIC EXTRACTS OF FOUR NATIVE MEDICINAL PLANTS OF ARDEBIL PROVINCE BY TWO METHODS OF DISK AND WELL DIFFUSION

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NARIMANI RASOUL; MOGHADDAM MOHAMMAD

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Journal: Research in Medicine Year:2016 | Volume:40 | Issue:3 Page(s): 149-154

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Title

INVESTIGATION OF ANTIBACTERIAL PROPERTIES OF ETHANOLIC EXTRACTS OF FOUR NATIVE MEDICINAL PLANTS OF ARDEBIL PROVINCE BY TWO METHODS OF DISK AND WELL DIFFUSION

Author(s)

NARIMANI RASOUL | MOGHADDAM MOHAMMAD | Issue Writer Certificate

Keywords

ANTIBACTERIAL PROPERTYQ1

EXTRACTQ2

DISK DIFFUSIONQ1

WELL DIFFUSIONQ1

MEDICINAL PLANTQ1

Abstract

Background: The main problems in antibiotic treatment are resistance to antibiotic and also the side effects of drug consumption. Therefore, after doing researches on the plants properties, humankind has attempted to use them in different industries. In this investigation the antimicrobial effects of ethanolic EXTRACTs of four therapeutic plants from Ardebil province include Nepeta nuda, Salvia verticilata, Nepeta crassifolia and Teocrium chamaedry subsp. syspirense, on three species of gram negative bacteria (Salmonella enteric, Escherichia coli, Pectobacterium carotovorum) were evaluated.Material and Methods: An experimental study was conducted at in vitro conditions. After preparing ethanolic EXTRACT of mentioned plants by maceration method, the antimicrobial effect of outcome EXTRACTs from their aerial parts were investigated by two different methods: DISK DIFFUSION and WELL DIFFUSION.Results: The highest growth inhibition zone in E. coli, P. caratovorum, S. enterica bacteria at 800 mL/mg concentration in DISK DIFFUSION method (12, 12, 12 mm) at N. crassifolia, N. nuda, and N. crassifolia EXTRACTs and in WELL DIFFUSION method (10, 12 and 11 mm) at S. verticilata, N. nuda and N. crassifolia EXTRACTs were seen respectively.Conclusion: It seems the different concentrations of EXTRACT and also bacterium type are influenced on growth inhibition zone. With increasing concentration, the inhibition zone is increased. In comparing to Gentamicin the EXTRACTs of mentioned plants had low effect on gram negative bacteria.

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APA: Copy

NARIMANI, RASOUL, & MOGHADDAM, MOHAMMAD. (2016). INVESTIGATION OF ANTIBACTERIAL PROPERTIES OF ETHANOLIC EXTRACTS OF FOUR NATIVE MEDICINAL PLANTS OF ARDEBIL PROVINCE BY TWO METHODS OF DISK AND WELL DIFFUSION. PAJOUHESH DAR PEZESHKI, 40(3 ), 149-154. SID. https://sid.ir/paper/41888/en

Vancouver: Copy

NARIMANI RASOUL, MOGHADDAM MOHAMMAD. INVESTIGATION OF ANTIBACTERIAL PROPERTIES OF ETHANOLIC EXTRACTS OF FOUR NATIVE MEDICINAL PLANTS OF ARDEBIL PROVINCE BY TWO METHODS OF DISK AND WELL DIFFUSION. PAJOUHESH DAR PEZESHKI[Internet]. 2016;40(3 ):149-154. Available from: https://sid.ir/paper/41888/en

IEEE: Copy

RASOUL NARIMANI, and MOHAMMAD MOGHADDAM, “INVESTIGATION OF ANTIBACTERIAL PROPERTIES OF ETHANOLIC EXTRACTS OF FOUR NATIVE MEDICINAL PLANTS OF ARDEBIL PROVINCE BY TWO METHODS OF DISK AND WELL DIFFUSION,” PAJOUHESH DAR PEZESHKI, vol. 40, no. 3 , pp. 149–154, 2016, [Online]. Available: https://sid.ir/paper/41888/en

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