DETECTION OF LISTERIA MONOCYTOGENES IN MILK BY THE POLYMERASE CHAIN REACTION

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SALMANZADEH AHRABI S.; REZAEI HEMAMI M.; ZALI MOHAMMAD REZA

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Journal: Research in Medicine Year:2004 | Volume:28 | Issue:3 Page(s): 211-214

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Title

DETECTION OF LISTERIA MONOCYTOGENES IN MILK BY THE POLYMERASE CHAIN REACTION

Author(s)

SALMANZADEH AHRABI S. | REZAEI HEMAMI M. | ZALI MOHAMMAD REZA | Issue Writer Certificate

Keywords

LISTERIA MONOCYTOGENESISQ2

MILKQ4

PCRQ3

Abstract

Background: Listeria monocytogenes may be the cause of meningitis and sepsis. It is transmitted via food products. Precise detection of this organism would undoubtedly play a significant role in prevention of this infection.Materials and methods: Polymerase chain reaction method was developed for detection of Listeria monocytogenes in MILK samples after enrichment culture. It consists of culturing samples in Listeria enrichment broth, followed by DNA extraction and detection of the organism using PCR.Results: Dilutions of Listeria monocytogenes in MILK were subjected to PCR amplification after enrichment culture. When determining the sensitivity of the method, it was found to be possible to detect 37 colony forming unit of the bacterium in MILK. When this PCR method was applied to extract of DNA from different bacteria no PCR products were observed.Conclusion: The method was assessed as a sensitive, specific and time-saving way of detecting L. monocytogenes in MILK samples.

Cites

FREQUENCY OF ACUTE ROTA-VIRAL DIARRHEA IN CHILDREN UNDER 6 YEARS IN GORGAN, IN 2006

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APA: Copy

SALMANZADEH AHRABI, S., REZAEI HEMAMI, M., & ZALI, MOHAMMAD REZA. (2004). DETECTION OF LISTERIA MONOCYTOGENES IN MILK BY THE POLYMERASE CHAIN REACTION. PAJOUHESH DAR PEZESHKI, 28(3), 211-214. SID. https://sid.ir/paper/42151/en

Vancouver: Copy

SALMANZADEH AHRABI S., REZAEI HEMAMI M., ZALI MOHAMMAD REZA. DETECTION OF LISTERIA MONOCYTOGENES IN MILK BY THE POLYMERASE CHAIN REACTION. PAJOUHESH DAR PEZESHKI[Internet]. 2004;28(3):211-214. Available from: https://sid.ir/paper/42151/en

IEEE: Copy

S. SALMANZADEH AHRABI, M. REZAEI HEMAMI, and MOHAMMAD REZA ZALI, “DETECTION OF LISTERIA MONOCYTOGENES IN MILK BY THE POLYMERASE CHAIN REACTION,” PAJOUHESH DAR PEZESHKI, vol. 28, no. 3, pp. 211–214, 2004, [Online]. Available: https://sid.ir/paper/42151/en

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