DIAGNOSIS OF BACTERIAL ENDOPHTHALMITIS BY NESTED- PCR IN COMPARISON WITH ROUTINE MICROBIOLOGICAL CULTURE

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ABD ALMALEKI A.R.; NASERPOUR FARIVAR T.; TAVALALI A.

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Journal: BINA Year:2005 | Volume:10 | Issue:2 (38) Page(s): 207-213

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Title

DIAGNOSIS OF BACTERIAL ENDOPHTHALMITIS BY NESTED- PCR IN COMPARISON WITH ROUTINE MICROBIOLOGICAL CULTURE

Author(s)

ABD ALMALEKI A.R. | NASERPOUR FARIVAR T. | TAVALALI A. | Issue Writer Certificate

Keywords

BACTERIAL ENDOPHTHALMITISQ3

NESTED POLYMERASE CHAIN REACTION (NESTED-PCR)Q3

Abstract

Purpose: To ascertain whether the use of nested-PCR (nested polymerase chain reaction) technique leads to more rapid and accurate diagnosis of BACTERIAL ENDOPHTHALMITIS.Methods: The aqueous humor and vitreous samples of 24 eyes of 24 patients with BACTERIAL ENDOPHTHALMITIS were evaluated by microscopy, diagnostic aerobic culture, and Nested- PCR to detect the infectious agent.Results: Aerobic cultures of the aqueous humor and the vitreous were positive in 1 eye (4%) and6 eyes (25%), respectively. Using Nested-PCR, an infectious agent was detected in the aqueous hurnor of 14 eyes (58%) and in the vitreous of 17 eyes (70%). Nested-PCR for eubacterial genome showed 100% correlation with bacteriologically positive specimens. Eubacterial genome was detected in 11 of 18 bacteriologically negative cultures of the vitreous.Conclusions: Nested-PCR on aqueous humor and vitreous samples is a powerful and reliable tool for the diagnosis of BACTERIAL ENDOPHTHALMITIS particularly in smear and culture negative specimens.

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APA: Copy

ABD ALMALEKI, A.R., NASERPOUR FARIVAR, T., & TAVALALI, A.. (2005). DIAGNOSIS OF BACTERIAL ENDOPHTHALMITIS BY NESTED- PCR IN COMPARISON WITH ROUTINE MICROBIOLOGICAL CULTURE. BINA, 10(2 (38)), 207-213. SID. https://sid.ir/paper/42246/en

Vancouver: Copy

ABD ALMALEKI A.R., NASERPOUR FARIVAR T., TAVALALI A.. DIAGNOSIS OF BACTERIAL ENDOPHTHALMITIS BY NESTED- PCR IN COMPARISON WITH ROUTINE MICROBIOLOGICAL CULTURE. BINA[Internet]. 2005;10(2 (38)):207-213. Available from: https://sid.ir/paper/42246/en

IEEE: Copy

A.R. ABD ALMALEKI, T. NASERPOUR FARIVAR, and A. TAVALALI, “DIAGNOSIS OF BACTERIAL ENDOPHTHALMITIS BY NESTED- PCR IN COMPARISON WITH ROUTINE MICROBIOLOGICAL CULTURE,” BINA, vol. 10, no. 2 (38), pp. 207–213, 2005, [Online]. Available: https://sid.ir/paper/42246/en

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