BIOREMEDIATION OF N-HEXADECANE CONTAMINATED SOILS USING PSEUDOMONAS AERUGINOSA BACTERIA ISOLATED FROM COASTAL AREAS

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TAYYBI TAHERH; JORFI SAHAND; GHAFFARI SHOKUH; KUJLU RAHELEH

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Title

BIOREMEDIATION OF N-HEXADECANE CONTAMINATED SOILS USING PSEUDOMONAS AERUGINOSA BACTERIA ISOLATED FROM COASTAL AREAS

Author(s)

TAYYBI TAHERH | JORFI SAHAND | GHAFFARI SHOKUH | KUJLU RAHELEH | Issue Writer Certificate

Keywords

BIOREMEDIATIONQ2

SOIL POLLUTIONQ2

N-HEXADECANEQ1

PSEUDOMONAS AERUGINOSAQ1

Abstract

Background and purpose: SOIL POLLUTION to hydrocarbons in oil field zones in southern Iran and areas close to oil and gas transportation pipe lines is a major environmental problem. The current study aimed at determining the efficiency of BIOREMEDIATION of N-HEXADECANE contaminated soil as a model contaminant.Materials and methods: Aged hydrocarbon contaminated soils were used as the origin of bacteria isolation. Isolation of N-HEXADECANE degrading bacteria was carried out through enrichment in mineral salt medium and incubation in 31 oC in shaker incubator at 150 rpm by repetitive cultures during 6 consecutive refreshing. Finally, two pure strains were isolated and identified by PCR. BIOREMEDIATION of initial N-HEXADECANE concentrations of 500, 1000 and 5000 mg/kg was investigated in eight weeks.Results: Two PSEUDOMONAS AERUGINOSA strains capable of N-HEXADECANE biodegradation were isolated. The removal efficiencies at the end of day 56 for N-HEXADECANE initial concentrations of 500, 1000 and 5000 mg/kg were 93.6%, 87.5%, and 69.62%, respectively.Conclusion: Isolated PSEUDOMONAS AERUGINOSA bacteria demonstrated high N-HEXADECANE biodegradation potential in soil and also biosurfactant production, therefore, they could be used for BIOREMEDIATION of hydrocarbon contaminated soils.

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APA: Copy

TAYYBI, TAHERH, JORFI, SAHAND, GHAFFARI, SHOKUH, & KUJLU, RAHELEH. (2016). BIOREMEDIATION OF N-HEXADECANE CONTAMINATED SOILS USING PSEUDOMONAS AERUGINOSA BACTERIA ISOLATED FROM COASTAL AREAS. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 26(140), 127-136. SID. https://sid.ir/paper/44472/en

Vancouver: Copy

TAYYBI TAHERH, JORFI SAHAND, GHAFFARI SHOKUH, KUJLU RAHELEH. BIOREMEDIATION OF N-HEXADECANE CONTAMINATED SOILS USING PSEUDOMONAS AERUGINOSA BACTERIA ISOLATED FROM COASTAL AREAS. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;26(140):127-136. Available from: https://sid.ir/paper/44472/en

IEEE: Copy

TAHERH TAYYBI, SAHAND JORFI, SHOKUH GHAFFARI, and RAHELEH KUJLU, “BIOREMEDIATION OF N-HEXADECANE CONTAMINATED SOILS USING PSEUDOMONAS AERUGINOSA BACTERIA ISOLATED FROM COASTAL AREAS,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 26, no. 140, pp. 127–136, 2016, [Online]. Available: https://sid.ir/paper/44472/en

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