KINETIC ACTIVITY OF ISOLATED NITRIC-OXIDE SYNTHASE ENZYME FROM SHEEP KIDNEY

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KADKHODAEI ELYADERANI MANIZHEH; MALEK ASKAR ALI MOHAMMAD; ROSTAMI MORAD

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2012 | Volume:22 | Issue:96 Page(s): 0-0

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Title

KINETIC ACTIVITY OF ISOLATED NITRIC-OXIDE SYNTHASE ENZYME FROM SHEEP KIDNEY

Author(s)

KADKHODAEI ELYADERANI MANIZHEH | MALEK ASKAR ALI MOHAMMAD | ROSTAMI MORAD | Issue Writer Certificate

Keywords

NITRIC OXIDE SYNTHASE (NOS)Q4

KINETIC ACTIVITYQ4

SHEEPQ4

KIDNEYQ4

Abstract

Background and purpose: Nitric oxide (NO) is produced by nitric oxide synthase (NOS) from L-arginine and has an important role in a variety of physiological and pathological processes in biological systems. In this study, kinetic activities of isolated NOS were evaluated from SHEEP KIDNEY.Materials and methods: In this research homogenization, ammonium sulfate precipitation and column chromatography on DEAE-32 Cellulose and 2', 5' -ADP-agarose of 500 grams of SHEEP KIDNEY were used to purify isolated nitric oxide. In all steps of purification process the amount of protein and its activity was assayed using Bradford and Griess reactions.Results: The molecular weight of SHEEP KIDNEY on SDS-PAGE electrophoresis was 54 KD.Specific activity was 0.6 units/mg protein and recovery of purification was 0.9% (relative purity was 20 times more). Optimum temperature and pH were 30oC and 7.4 and incubation of purified enzyme at thermal interval of 10 to 30oC for 15 minute showed a stable enzyme activity. At 75mM concentration of L-arginine substrate, the highest level of NOS activity was seen. The Vmax and Km of enzyme were 250 nmol/min/mg protein and 5.32mM, respectively.Conclusion: NOS enzyme from SHEEP KIDNEY was purified with relative purity of 20 times and their optimum temperature, pH, suitable substrate concentration, Vmax and Km were 30oC, 7.4, 75mM, 250 nmol/min/mg proteins and 5.32mmol, respectively.

Cites

EVALUATION OF ORGANOPHOSPHORUS PESTICIDE DIAZINON RESIDUES IN GREENHOUSE CROPS (CASE STUDY: ZUCCHINI)

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APA: Copy

KADKHODAEI ELYADERANI, MANIZHEH, MALEK ASKAR, ALI MOHAMMAD, ROSTAMI, MORAD, ABEROMAND, MOHAMMAD, & KHIROLLAH, ALIREZA. (2012). KINETIC ACTIVITY OF ISOLATED NITRIC-OXIDE SYNTHASE ENZYME FROM SHEEP KIDNEY. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 22(96), 0-0. SID. https://sid.ir/paper/44622/en

Vancouver: Copy

KADKHODAEI ELYADERANI MANIZHEH, MALEK ASKAR ALI MOHAMMAD, ROSTAMI MORAD, ABEROMAND MOHAMMAD, KHIROLLAH ALIREZA. KINETIC ACTIVITY OF ISOLATED NITRIC-OXIDE SYNTHASE ENZYME FROM SHEEP KIDNEY. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2012;22(96):0-0. Available from: https://sid.ir/paper/44622/en

IEEE: Copy

MANIZHEH KADKHODAEI ELYADERANI, ALI MOHAMMAD MALEK ASKAR, MORAD ROSTAMI, MOHAMMAD ABEROMAND, and ALIREZA KHIROLLAH, “KINETIC ACTIVITY OF ISOLATED NITRIC-OXIDE SYNTHASE ENZYME FROM SHEEP KIDNEY,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 22, no. 96, pp. 0–0, 2012, [Online]. Available: https://sid.ir/paper/44622/en

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