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Information Journal Paper

Title

EVALUATION SPREAD OF ANT (2”)-IA GENE IN AMINOGLYCOSIDE RESISTANT ESCHERICHIA COLI ISOLATED FROM URINE BY PCR

Pages

  175-182

Keywords

ANT (2”)-IAQ2
UTIQ3

Abstract

 Background and purpose: ESCHERICHIA COLI is the most prevalent etiologic agent of urinary tract infection. Enzymatic inactivation of aminoglycosides by aminoglycoside-modifying enzymes is the main mechanism of resistance to these antibiotics in ESCHERICHIA COLI. The aim of this study was detection of ant (2”)-Ia gene among aminoglycoside resistant clinical isolates of E. coli using PCR methodMaterials and methods: 276 clinical isolates of E. coli were collected, antibiotic susceptibility patterns of isolates were determined by disk diffusion method for gentamicin, amikacin, tobramycin, kanamycin and netilmicin paper disks considering CLSI principles. Chromosomal DNA of the isolates was extracted using DNA extraction kits and PCR method was used for detection of the ant (2”)-Ia geneResults: Results of disk diffusion showed that 24.63%, 23.18%, 21.01%, 6.15% and 3.62% of E. coli isolates were resistant to tobramycin, kanamycin, gentamicin, netilmicin and amikacin, respectively. Ant (2”)-Ia gene was detected in 47.88% of E. coli isolatesConclusion: Because of high prevalence of resistance toward aminoglycoside antibiotics due to its transfer among bacteria by transferable elements such as transposons and plasmids, tracing of transfer routs among different bacteria is very important.

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  • Cite

    APA: Copy

    SOLEYMANI, NEDA, SATARI, MORTEZA, BRUMAND, MOHAMAD ALI, & MOHABATI MOBAREZ, ASHRAF. (2012). EVALUATION SPREAD OF ANT (2”)-IA GENE IN AMINOGLYCOSIDE RESISTANT ESCHERICHIA COLI ISOLATED FROM URINE BY PCR. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 21(SUPPLEMENT 1), 175-182. SID. https://sid.ir/paper/44749/en

    Vancouver: Copy

    SOLEYMANI NEDA, SATARI MORTEZA, BRUMAND MOHAMAD ALI, MOHABATI MOBAREZ ASHRAF. EVALUATION SPREAD OF ANT (2”)-IA GENE IN AMINOGLYCOSIDE RESISTANT ESCHERICHIA COLI ISOLATED FROM URINE BY PCR. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2012;21(SUPPLEMENT 1):175-182. Available from: https://sid.ir/paper/44749/en

    IEEE: Copy

    NEDA SOLEYMANI, MORTEZA SATARI, MOHAMAD ALI BRUMAND, and ASHRAF MOHABATI MOBAREZ, “EVALUATION SPREAD OF ANT (2”)-IA GENE IN AMINOGLYCOSIDE RESISTANT ESCHERICHIA COLI ISOLATED FROM URINE BY PCR,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 21, no. SUPPLEMENT 1, pp. 175–182, 2012, [Online]. Available: https://sid.ir/paper/44749/en

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