QUANTITATIVE AND QUALITATIVE INVESTIGATION ON PYRROLYZIDINE ALKALOIDS IN ROOTS, LEAVES, PETALS AND SEEDS OF IRANIAN ECHIUM AMOENUM FISCH. & MEY.

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AZADBAKHT MOHAMAD; NEMATZADEH GHORBAN ALI; HOSSEINPOUR AZAD NURODIN; SHOKRI EHSAN

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Paper Information

Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2012 | Volume:21 | Issue:SUPPLEMENT 1 Page(s): 131-137

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Title

QUANTITATIVE AND QUALITATIVE INVESTIGATION ON PYRROLYZIDINE ALKALOIDS IN ROOTS, LEAVES, PETALS AND SEEDS OF IRANIAN ECHIUM AMOENUM FISCH. & MEY.

Author(s)

AZADBAKHT MOHAMAD | NEMATZADEH GHORBAN ALI | HOSSEINPOUR AZAD NURODIN | SHOKRI EHSAN | Issue Writer Certificate

Keywords

ECHIUM AMOENUMQ2

PYROLIZIDINE ALKALOEIDQ2

SENECIONINQ2

SPECTROPHOTOMETRYQ2

Abstract

Background and purpose: Pyrrolizidin alkaloeids (PAs) are a main gorupes of plant toxin that 200 types of these materials had been identified at yet. Plants containing these alkaloeids are known to be significant causes of dead and desease in mammals such as humans. This research were conducted to assesment of PAs quality and quantity in different organs of iranian ECHIUM AMOENUM.Materials and methods: PAs presence were considered in root, petal, leaves and seed by erlich reagent. Color apparence in extracts solation approves that PAs exists in organs. Also, regarding SENECIONIN in samples extract, the quantity of PAs and these N-oxide were determined.This method is specific for alkaloids and other components with a non- basic unsaturated part (D- Pirolin ring).Results: Collection of PAs and N-oxide stimated in 500 mg of sample was 0.031-0.053 mg for root, 0.369mg for leaves, 0.026 mg for petals and not significantly detected in seeds.Conclusion: Compaired to SENECIONIN LD50 (64.12±2.24 mg/kg) and pyrroliziding alkaloeids lethal doses (2-27 mg/kg), leaf samples of Iranian ECHIUM AMOENUM extracts are able to make toxical condition, unlike seed, petal and root samples PAs which not able to be toxic, although using these material for long period, even at low level, can be dangerouse to body organs and cause hepatotoxical desease.

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