DIAGNOSIS OF STREPTOCOCCUS PNEUMONIAE BASED ON CPSB, CPSD, AND PSAA GENES

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ASGARI ALI; ATAEE RAMEZAN ALI; GHORBANALIZADEGAN MAHDI; MEHRABI TAVANA ALI

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2018 | Volume:28 | Issue:161 Page(s): 121-126

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Title

DIAGNOSIS OF STREPTOCOCCUS PNEUMONIAE BASED ON CPSB, CPSD, AND PSAA GENES

Author(s)

ASGARI ALI | ATAEE RAMEZAN ALI | GHORBANALIZADEGAN MAHDI | MEHRABI TAVANA ALI | Issue Writer Certificate

Keywords

PCRQ1

STREPTOCOCCUS PNEUMONIAEQ2

CAPSULAR GENESQ1

Abstract

Background and purpose: Fast diagnosis of pneumococcal infections is of great importance inachieving successful outcomes. The aim of this study was to use PCR process based on capsular encodinggenes of Streptococcus pneumonia. Materials and methods: In this experimental study, specific primers were designed based onthe reference sequences of the STREPTOCOCCUS PNEUMONIAE cpsB, cpsD, aroE, and psaA genes. All bacteriawere subjected to DNA extraction separately. Then, the PCR protocol was performed. Sequencing of thePCR products was done and data was analyzed descriptively. Results: The primer pairs against the cpsB, cpsD, aroE, and psaA genes amplified 159, 202, 254, and 286 bp fragments, respectively. The alignments and comparison of amplified sequences showed ahigh homology (100%) with the reference genes. Conclusion: According to this study, it is possible to diagnose STREPTOCOCCUS PNEUMONIAE basedon virulence genes, and differentiate the pathogenic STREPTOCOCCUS PNEUMONIAE from nonpathogenic strainsand also viridans groups. Therefore, this method is suggested in diagnosis of pathogenic Streptococcuspneumoniae strains.

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APA: Copy

ASGARI, ALI, ATAEE, RAMEZAN ALI, GHORBANALIZADEGAN, MAHDI, & MEHRABI TAVANA, ALI. (2018). DIAGNOSIS OF STREPTOCOCCUS PNEUMONIAE BASED ON CPSB, CPSD, AND PSAA GENES. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 28(161 ), 121-126. SID. https://sid.ir/paper/45471/en

Vancouver: Copy

ASGARI ALI, ATAEE RAMEZAN ALI, GHORBANALIZADEGAN MAHDI, MEHRABI TAVANA ALI. DIAGNOSIS OF STREPTOCOCCUS PNEUMONIAE BASED ON CPSB, CPSD, AND PSAA GENES. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;28(161 ):121-126. Available from: https://sid.ir/paper/45471/en

IEEE: Copy

ALI ASGARI, RAMEZAN ALI ATAEE, MAHDI GHORBANALIZADEGAN, and ALI MEHRABI TAVANA, “DIAGNOSIS OF STREPTOCOCCUS PNEUMONIAE BASED ON CPSB, CPSD, AND PSAA GENES,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 28, no. 161 , pp. 121–126, 2018, [Online]. Available: https://sid.ir/paper/45471/en

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