ACTIVITY OF AVICENNIA MARINA METHANOL EXTRACTS ON PROLIFERATION OF LYMPHOCYTES AND THEIR MUTAGENICITY USING AMES TEST AND IN SILICO METHOD

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KHAJEHZADEH SARA; BEHBAHANI MANDANA

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Journal: Journal of Mazandaran University of Medical Sciences Year:2016 | Volume:26 | Issue:135 Page(s): 32-42

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Title

ACTIVITY OF AVICENNIA MARINA METHANOL EXTRACTS ON PROLIFERATION OF LYMPHOCYTES AND THEIR MUTAGENICITY USING AMES TEST AND IN SILICO METHOD

Author(s)

KHAJEHZADEH SARA | BEHBAHANI MANDANA | Issue Writer Certificate

Keywords

AVICENNIAQ2

LYMPHOCYTESQ3

METHANOLIC EXTRACTQ2

MTT ASSAYQ2

MUTAGENICITYQ3

Abstract

Background and purpose: AVICENNIA marina (family Acanthaceae) has been used as traditional medicine in Iran to treat some diseases such as ulcers, rheumatism and burns. The present study investigated the in silico and in vitro MUTAGENICITY of the fruit, leaf, seed and stem extracts of AVICENNIA marina and their effects on human peripheral blood mononuclear cells (PBMC) proliferation.Materials and methods: In this experimental study, air dried and powdered plant materials were extracted by methanol using maceration. The extracts were evaporated to dryness by rotary evaporator at 40oC and were diluted using different PBS concentrations (50, 100, 500, 1000, and 1500 mg/ml). The effect of the methanol extract of this plant on lymphocyte proliferation was measured using MTT ASSAY. The MUTAGENICITY of these extracts was also investigated using Ames test. In silico analysis of 15 dominant compounds of the plant was performed by Toxtree 2.6.6 software.Results: The methanol extracts of the leaf and root had the highest and lowest inducing effect on lymphocyte proliferation, respectively. Leaf and stem extracts of AVICENNIA marina did not show any MUTAGENICITY on this strain. In silico analysis demonstrated that among 15 compounds, four triterpenoids (Taraxerol, Betulin, Lupeol, and Gossypol) had weak MUTAGENICITY.Conclusion: AVICENNIA marina showed positive effects on proliferation of LYMPHOCYTES and their MUTAGENICITY, therefore, it could be considered as a good candidate in treatment of immunodeficiency diseases.

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APA: Copy

KHAJEHZADEH, SARA, & BEHBAHANI, MANDANA. (2016). ACTIVITY OF AVICENNIA MARINA METHANOL EXTRACTS ON PROLIFERATION OF LYMPHOCYTES AND THEIR MUTAGENICITY USING AMES TEST AND IN SILICO METHOD. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 26(135), 32-42. SID. https://sid.ir/paper/45704/en

Vancouver: Copy

KHAJEHZADEH SARA, BEHBAHANI MANDANA. ACTIVITY OF AVICENNIA MARINA METHANOL EXTRACTS ON PROLIFERATION OF LYMPHOCYTES AND THEIR MUTAGENICITY USING AMES TEST AND IN SILICO METHOD. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;26(135):32-42. Available from: https://sid.ir/paper/45704/en

IEEE: Copy

SARA KHAJEHZADEH, and MANDANA BEHBAHANI, “ACTIVITY OF AVICENNIA MARINA METHANOL EXTRACTS ON PROLIFERATION OF LYMPHOCYTES AND THEIR MUTAGENICITY USING AMES TEST AND IN SILICO METHOD,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 26, no. 135, pp. 32–42, 2016, [Online]. Available: https://sid.ir/paper/45704/en

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