POLYMORPHIC MARKERS LOCATED ON Y-CHROMOSOME FROM THE POPULATION OF EASTERN-AZERBAIJAN

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BONYADI MORTAZA; ABHARI MASOUMEH; OMRANI OMID; EMAMALIZADEH BABAK

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Journal: MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES Year:2010 | Volume:32 | Issue:4 Page(s): 16-20

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Title

POLYMORPHIC MARKERS LOCATED ON Y-CHROMOSOME FROM THE POPULATION OF EASTERN-AZERBAIJAN

Author(s)

BONYADI MORTAZA | ABHARI MASOUMEH | OMRANI OMID | EMAMALIZADEH BABAK | Issue Writer Certificate

Keywords

MICROSATELLITESQ3

CHROMOSOME YQ3

EASTERN AZERBAIJAN POPULATIONQ3

Abstract

Background and Objectives: CHROMOSOME Y is a sex-determinant chromosome in human.MICROSATELLITES located on this chromosome are being applied for paternity test, sex determinations in neonates and DNA finger-printing. The application of these markers in most cases depends on the polymorphic situation of them. The aim of this study was to study some of Polymorphic these markers located on Y chromosome among male population from Eastern- Azerbaijan.Materials and Methods: Fifty unrelated males and several females as a negative control were randomly selected from the interested population. DNA from whole blood cells were extracted and all the individuals were studied by three pairs MICROSATELLITES (DYS438, DYS390, DYS385) selected from the CHROMOSOME Y. PCR products were electrophoresed on ploy-acrylamid gel (SSR-PCR technique) and bands were analyzed.Results: None of the studied markers were amplified in female controls indicating that these markers are male specific and they do not have a copy on autosomal or X chromosomes.DYS385, a poly-allelic marker, showed the highest percentage of polymorphism (95%) in this population. Markers DYS435 and DYS390 showed relatively less polymorphism respectively.Conclusion: This result indicates that in the population of East Azerbaijan DYS385 is one of the best markers for paternity test and DNA finger printing. In case of combination use of this marker with other two markers, the efficiency of the result would be highly increased.

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APA: Copy

BONYADI, MORTAZA, ABHARI, MASOUMEH, OMRANI, OMID, & EMAMALIZADEH, BABAK. (2010). POLYMORPHIC MARKERS LOCATED ON Y-CHROMOSOME FROM THE POPULATION OF EASTERN-AZERBAIJAN. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, 32(4), 16-20. SID. https://sid.ir/paper/46817/en

Vancouver: Copy

BONYADI MORTAZA, ABHARI MASOUMEH, OMRANI OMID, EMAMALIZADEH BABAK. POLYMORPHIC MARKERS LOCATED ON Y-CHROMOSOME FROM THE POPULATION OF EASTERN-AZERBAIJAN. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2010;32(4):16-20. Available from: https://sid.ir/paper/46817/en

IEEE: Copy

MORTAZA BONYADI, MASOUMEH ABHARI, OMID OMRANI, and BABAK EMAMALIZADEH, “POLYMORPHIC MARKERS LOCATED ON Y-CHROMOSOME FROM THE POPULATION OF EASTERN-AZERBAIJAN,” MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, vol. 32, no. 4, pp. 16–20, 2010, [Online]. Available: https://sid.ir/paper/46817/en

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