STUDY OF PH GRADIENT ON LIPOSOMES TRAPPING EFFICIENCIES BY SPECTROPHOTOMETRY: USING NEUTRAL RED

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MOHAMMADI BARDBORI A.

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Journal: PHARMACEUTICAL SCIENCES Year:2006 | Volume:- | Issue:2 Page(s): 27-31

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Title

STUDY OF PH GRADIENT ON LIPOSOMES TRAPPING EFFICIENCIES BY SPECTROPHOTOMETRY: USING NEUTRAL RED

Author(s)

MOHAMMADI BARDBORI A. | Issue Writer Certificate

Keywords

LIPOSOMEQ2

MOLECULAR NRQ2

IONIC NRQ2

TRAPPING EFFICIENCYQ2

SPHECTOPHOTOMETRYQ2

Abstract

Objectives: The purpose of this study was to establish a new experimental, rapid, simple and cost effective approach to study LIPOSOMEs. NR (Neutral red) was selected as a model of lipophilic and hydrophilic drug. NR is an indicator of pH and a weak base with pka=6.7 which is red while ionized in acidic media but yellow while non-ionized in alkaline media and is used in vital staining. Methods: In this study the effects of pH internal (5) and external media (5- 9) on LIPOSOMEs trapping efficiencies in wavelength of 533nm were examined. Results: %EE is (the entrapment efficiency of LIPOSOME) increased between (%24-%87) in pH (external media) range 5-9. CBC (CBC, contrast between liposomal compartment for visualized by LM) and EBC (EBC, exchanged between liposomal compartment) and EE were calculated. LIPOSOMEs with the internal (pH = 5) and external media (pH = 9) is the most suitable option for staining with NR. No significant correlations were observed among the two index with a 95% coefficient interval (EBC with CBC, r2 = 0.93, p<0.05, and EE with CBC r2=0.93, p<0.05). Significant correlations were also observed among the EBC index and EE index with a 95% coefficient interval (EBC with EE r2=0.66, p<0.05). Conclusion: NR accumulates in LIPOSOMEs in ionized form therefore, giving sharp contrast with the surrounding media of LIPOSOMEs. This makes them to be visualized by LM (light microscope).

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APA: Copy

MOHAMMADI BARDBORI, A.. (2006). STUDY OF PH GRADIENT ON LIPOSOMES TRAPPING EFFICIENCIES BY SPECTROPHOTOMETRY: USING NEUTRAL RED. PHARMACEUTICAL SCIENCES, -(2), 27-31. SID. https://sid.ir/paper/48453/en

Vancouver: Copy

MOHAMMADI BARDBORI A.. STUDY OF PH GRADIENT ON LIPOSOMES TRAPPING EFFICIENCIES BY SPECTROPHOTOMETRY: USING NEUTRAL RED. PHARMACEUTICAL SCIENCES[Internet]. 2006;-(2):27-31. Available from: https://sid.ir/paper/48453/en

IEEE: Copy

A. MOHAMMADI BARDBORI, “STUDY OF PH GRADIENT ON LIPOSOMES TRAPPING EFFICIENCIES BY SPECTROPHOTOMETRY: USING NEUTRAL RED,” PHARMACEUTICAL SCIENCES, vol. -, no. 2, pp. 27–31, 2006, [Online]. Available: https://sid.ir/paper/48453/en

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