IDENTIFICATION, ISOLATION, CLONING AND SEQUENCING OF FLUCONAZOLE RESISTANCE GENE IN ASPERGILLUS FUMIGATUS

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KAZEMI ABD ALHASAN; ROBSON G.D.; DENNING D.W.; ASGHARZADEH M.

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Journal: PHARMACEUTICAL SCIENCES Year:2008 | Volume:- | Issue:2 Page(s): 27-35

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Title

IDENTIFICATION, ISOLATION, CLONING AND SEQUENCING OF FLUCONAZOLE RESISTANCE GENE IN ASPERGILLUS FUMIGATUS

Author(s)

KAZEMI ABD ALHASAN | ROBSON G.D. | DENNING D.W. | ASGHARZADEH M. | Issue Writer Certificate

Keywords

FLUCONAZOLE RESISTANCEQ2

GENEQ2

SEQUENCINGQ2

ASPERGILLUS FUMIGATUSQ2

Abstract

Objectives: Development of azole resistance in opportunistic fungi is the most problematic reason in immunocompromised patients and at present time, there is an increased awareness of the morbidity and mortality associated with fungal infections caused by resistant fungi to imidazole. This research was carried out for cloning and SEQUENCING of FLUCONAZOLE RESISTANCE GENE. Methods: Using genomic DNA of Aspergilus fumigatus (ATCC strain 90254) a primary 800 bp PCR product and then a secondary 1750 bp inverse PCR product were obtained. The 1750 bp PCR product was gel purified and cloned into E. coli using the suitable plasmid. Plasmid was extracted from transformed E. coli and the presence of expected insert into plasmid, was confirmed by digestion of plasmid using Eco R1 restriction enzyme. Also southern analysis using standard protocol was carried out for investigation of GENE expression. Results: Gel purified 1750 bp band was sequenced and after deletion of extra nucleotides from both side of residue a 1703 bp motif submitted at NCBI GENE bank with accession No.: AY848856. Conclusion: In conclusion considering (resistant to fluconazole) A. fumigatus resistance GENE to this compound was cloned and sequenced successfully in our lab. Since A. fumigatus is increasingly resistant to the widely used fluconazole, we emphasize for future additional studies for expression of this GENE and its mRNA transcription to understand the effective and inhibitor factors for expression of GENE.

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APA: Copy

KAZEMI, ABD ALHASAN, ROBSON, G.D., DENNING, D.W., & ASGHARZADEH, M.. (2008). IDENTIFICATION, ISOLATION, CLONING AND SEQUENCING OF FLUCONAZOLE RESISTANCE GENE IN ASPERGILLUS FUMIGATUS. PHARMACEUTICAL SCIENCES, -(2), 27-35. SID. https://sid.ir/paper/48463/en

Vancouver: Copy

KAZEMI ABD ALHASAN, ROBSON G.D., DENNING D.W., ASGHARZADEH M.. IDENTIFICATION, ISOLATION, CLONING AND SEQUENCING OF FLUCONAZOLE RESISTANCE GENE IN ASPERGILLUS FUMIGATUS. PHARMACEUTICAL SCIENCES[Internet]. 2008;-(2):27-35. Available from: https://sid.ir/paper/48463/en

IEEE: Copy

ABD ALHASAN KAZEMI, G.D. ROBSON, D.W. DENNING, and M. ASGHARZADEH, “IDENTIFICATION, ISOLATION, CLONING AND SEQUENCING OF FLUCONAZOLE RESISTANCE GENE IN ASPERGILLUS FUMIGATUS,” PHARMACEUTICAL SCIENCES, vol. -, no. 2, pp. 27–35, 2008, [Online]. Available: https://sid.ir/paper/48463/en

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