ISOLATION AND MOLECULAR IDENTIFICATION OF ACANTHAMOEBA IN SURFACE STAGNANT WATERS OF QAZVIN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HOSSEINBIGI B.; SARAIE SAHNEHSARAIE M.; ALIZADEH S.A.; RASTI S.; EFTAKHAR M.; KHOSROU SHAHI N.; HOOSHYAR HOSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Inflammatory Diseases Year:2012 | Volume:16 | Issue:3 (64) Page(s): 27-32

Download Full-Text

Persian Verion

View:

1,168

Download:

Cites:

Information Journal Paper

Title

ISOLATION AND MOLECULAR IDENTIFICATION OF ACANTHAMOEBA IN SURFACE STAGNANT WATERS OF QAZVIN

Author(s)

Keywords

ACANTHAMOEBAQ2

SURFACE STAGNANT WATERSQ2

AMOEBAQ2

PCRQ3

Abstract

Background: Regarding the increasing number of acanthamoebiasis cases in recent decades, investigating the environmental pollution of this AMOEBA is now a focus of more attention. Surface stagnant water is considered as one of the important sources of human infections.Objective: The aim of this study was isolation and molecular identification of ACANTHAMOEBA spp in SURFACE STAGNANT WATERS of Qazvin.Methods: This was a descriptive study carried out in the autumn of 2010. A total of 40 samples of SURFACE STAGNANT WATERS from the city parks and squares in Qazvin were collected. Samples were initially filtered using 0.45 m nitrocellulose membrane filters and later the residual components left on filter membrane cultured on non-nutrient agar. The cultures media were microscopically examined for the presence of trophozoites and cysts of free-living AMOEBAe. Positive cultures for AMOEBAe were examined by PCR (polymerase chain reaction) method using specific primers for the genus of ACANTHAMOEBA.Findings: Free-living AMOEBAe were identified in 32 (80%) samples by culture method. In addition, ACANTHAMOEBA was identified by PCR method in 14 (43.8%) cases of positive cultures showing a nearly 500bp band.Conclusion: considering the prevalent of ACANTHAMOEBA in SURFACE STAGNANT WATERS of Qazvin, more attention to the potential role of such waters in transmission of infection by the regional clinicians and health practitioners is necessary.

Cites

No record.

References

No record.

Cite

APA: Copy

HOSSEINBIGI, B., SARAIE SAHNEHSARAIE, M., ALIZADEH, S.A., RASTI, S., EFTAKHAR, M., KHOSROU SHAHI, N., & HOOSHYAR, HOSSEIN. (2012). ISOLATION AND MOLECULAR IDENTIFICATION OF ACANTHAMOEBA IN SURFACE STAGNANT WATERS OF QAZVIN. JOURNAL OF INFLAMMATORY DISEASES (THE JOURNAL OF QAZVIN UNIVERSITY OF MEDICAL SCIENCES), 16(3 (64)), 27-32. SID. https://sid.ir/paper/48964/en

Vancouver: Copy

HOSSEINBIGI B., SARAIE SAHNEHSARAIE M., ALIZADEH S.A., RASTI S., EFTAKHAR M., KHOSROU SHAHI N., HOOSHYAR HOSSEIN. ISOLATION AND MOLECULAR IDENTIFICATION OF ACANTHAMOEBA IN SURFACE STAGNANT WATERS OF QAZVIN. JOURNAL OF INFLAMMATORY DISEASES (THE JOURNAL OF QAZVIN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2012;16(3 (64)):27-32. Available from: https://sid.ir/paper/48964/en

IEEE: Copy

B. HOSSEINBIGI, M. SARAIE SAHNEHSARAIE, S.A. ALIZADEH, S. RASTI, M. EFTAKHAR, N. KHOSROU SHAHI, and HOSSEIN HOOSHYAR, “ISOLATION AND MOLECULAR IDENTIFICATION OF ACANTHAMOEBA IN SURFACE STAGNANT WATERS OF QAZVIN,” JOURNAL OF INFLAMMATORY DISEASES (THE JOURNAL OF QAZVIN UNIVERSITY OF MEDICAL SCIENCES), vol. 16, no. 3 (64), pp. 27–32, 2012, [Online]. Available: https://sid.ir/paper/48964/en

Related Journal Papers