DIAGNOSIS AND EVALUATION OF CAMPYLOBACTES BY PCR AND CULTURE

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HAVAEI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Journal of Isfahan Medical School Year:2002 | Volume:19 | Issue:63-64 Page(s): 61-66

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Title

DIAGNOSIS AND EVALUATION OF CAMPYLOBACTES BY PCR AND CULTURE

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HAVAEI A. | | | Issue Writer Certificate

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Abstract

Enteritis due to campylobacter is the most common cause of acute bacterial diarrhea worldwide. Eighteen species and subspecies of campylobacter have been described, two of these (C.jejuni and C.coli) account for the majority of human infections.Campylobacters are asaccharolytic, fastidious bacteria, and this limits the available biochemical tests by which isolates may by differentiated. These limitations might in principle be overcome by use of PCR technique. To compare the culture techniqne with PCR assay, a total of 196 fecal specimens from patients with symptoms of enteritis were tested for the presence of campylobacters using culture and PCR All the specimens were cultured on campylobacter selective agar and incubated in microaerophilic conditons at 42˚c for 48 - 72 h. Identification of isolated campylobacters to species level was done by following procedures: Gram stain, oxidase and catalase activities, hippurate hydrolysis, and susceptibility to nalidixic acid and cephalothin.DNA extraction from fecal samples was done by use of High Pure PCR Template Preparation Kit. The ploymerase chain reaction (PCR) was used to direct detection of thermophilic Campylobacter jejuni and Campylobacter coli in DNA extractions from fecaI samples. Two species - specific PCR primer sets (JEJ1, JEJ2, COL1, COL2) was used to differentiation between C.jejuni and C.coli in fecal samples. Compylobacter strains were isolated from 15 (7.6%) of 196 patients with diarrhea (12 C.jejuni and 3 C.coli). By contrast, PCR assay detected campylobacters in 18 of 196 samples, including 15 previous culture positive samples, and further identified them as C.jejuni (14) and C.coli (4). The sensitivity and specificity of PCR was 100 and 98% respectively. According to the results of present study, it is proposed that PCR is a reliable and sensitive method which may be used as a routine diagnostic technique for detection of compylobacters in clinical specimens.

Cites

CYTOLETHAL DISTENDING TOXIN (CDT) PRODUCED BY CAMPYLOBACTER JEJUNI AND CAMPYLOBACTER COLI ISOLATED FROM CHIKENS BY TISSUE CULTURE METHOD IN ISFAHAN

References

جستجوی کامپیلو باکتر در کودکان زیر 5 سال مبتلا به اسهال مراجعه کننده به بیمارستان تخصصی اطفال زاهدان در سال 1377

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APA: Copy

HAVAEI, A.. (2002). DIAGNOSIS AND EVALUATION OF CAMPYLOBACTES BY PCR AND CULTURE. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 19(63-64), 61-66. SID. https://sid.ir/paper/49748/en

Vancouver: Copy

HAVAEI A.. DIAGNOSIS AND EVALUATION OF CAMPYLOBACTES BY PCR AND CULTURE. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2002;19(63-64):61-66. Available from: https://sid.ir/paper/49748/en

IEEE: Copy

A. HAVAEI, “DIAGNOSIS AND EVALUATION OF CAMPYLOBACTES BY PCR AND CULTURE,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 19, no. 63-64, pp. 61–66, 2002, [Online]. Available: https://sid.ir/paper/49748/en

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