TRANSCRIPTOMIC EVALUATION OF CBFA1 GENE DURING OSTEOGENIC DIFFERENTIATION OF ADIPOSE DERIVED MESENCHYMAL STEM CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

DARAEI ABDOLREZA; GHAFARIZADEH ALI ASGHAR; HASHEMIBENI BATOUL; SALEHI RASOUL; SALEHI MOHAMMADI R.; BAHRAMBEIGI VAHID

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Isfahan Medical School Year:2013 | Volume:31 | Issue:236 Page(s): 640-648

Download Full-Text

Persian Verion

View:

642

Download:

Cites:

Information Journal Paper

Title

TRANSCRIPTOMIC EVALUATION OF CBFA1 GENE DURING OSTEOGENIC DIFFERENTIATION OF ADIPOSE DERIVED MESENCHYMAL STEM CELLS

Author(s)

Keywords

ADIPOSE DERIVED MESENCHYMAL STEM CELLQ2

CORE BINDING FACTOR ALPHA 1 (CBFA1)Q2

OSTEOBLASTQ2

Abstract

Background: Bone is a tissue with high repair capacity; but, this ability dramatically is limited during irrecoverable bone and skeletal defects. Stem cell therapy is a promising approach for construction of bone tissue. Mesenchymal stem cells (MSCs) have been introduced as basic tools for bone tissue generation in tissue engineering. Core binding factor alpha 1 (Cbfa1) is one of the main genes involved in osteogenesis. Since relative expression of Cbfa1 in differentiated OSTEOBLASTs from adipose derived stem cells is unknown, this study aimed to evaluate the gene expression pattern of Cbfa1 in adipose derived differentiated OSTEOBLASTs in 4 different time intervals.Methods: Real-time PCR was used for studying the gene expression of Cbfa1 in human normal OSTEOBLASTs and adipose derived osteogenic OSTEOBLASTs at the days 7, 14, 21 and 28.Findings: There was a progressive increase in Cbfa1 expression over the differentiation period of ADIPOSE DERIVED MESENCHYMAL STEM CELLs (ADSCs) from day 7 to day 28. Differentiated OSTEOBLASTs expressed Cbfa1 lower than native OSTEOBLASTs (P<0.05).Conclusion: ADSCs are an attractive source for engineering a new bone tissue. According to our results, adipose derived differentiated OSTEOBLASTs express candidate gene at least on day 7 of culture.Our study indicates that longer treatment of ADSCs in osteogenic medium confer better OSTEOBLASTic properties to these cells.

Cites

No record.

References

No record.

Cite

APA: Copy

DARAEI, ABDOLREZA, GHAFARIZADEH, ALI ASGHAR, HASHEMIBENI, BATOUL, SALEHI, RASOUL, SALEHI MOHAMMADI, R., & BAHRAMBEIGI, VAHID. (2013). TRANSCRIPTOMIC EVALUATION OF CBFA1 GENE DURING OSTEOGENIC DIFFERENTIATION OF ADIPOSE DERIVED MESENCHYMAL STEM CELLS. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 31(236), 640-648. SID. https://sid.ir/paper/50157/en

Vancouver: Copy

DARAEI ABDOLREZA, GHAFARIZADEH ALI ASGHAR, HASHEMIBENI BATOUL, SALEHI RASOUL, SALEHI MOHAMMADI R., BAHRAMBEIGI VAHID. TRANSCRIPTOMIC EVALUATION OF CBFA1 GENE DURING OSTEOGENIC DIFFERENTIATION OF ADIPOSE DERIVED MESENCHYMAL STEM CELLS. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2013;31(236):640-648. Available from: https://sid.ir/paper/50157/en

IEEE: Copy

ABDOLREZA DARAEI, ALI ASGHAR GHAFARIZADEH, BATOUL HASHEMIBENI, RASOUL SALEHI, R. SALEHI MOHAMMADI, and VAHID BAHRAMBEIGI, “TRANSCRIPTOMIC EVALUATION OF CBFA1 GENE DURING OSTEOGENIC DIFFERENTIATION OF ADIPOSE DERIVED MESENCHYMAL STEM CELLS,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 31, no. 236, pp. 640–648, 2013, [Online]. Available: https://sid.ir/paper/50157/en

Related Journal Papers