COMPARISON OF THE APOPTOSIS INDUCTION EFFECT OF POMEGRANATE PEEL EXTRACT ONHUMAN UMBILICAL VEIN ENDOTHELIAL CELLS AND MELANOMA

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DANA NASIM; HAGHJOOY JAVANMARD SHAGHAYEGH; RAFIEE LALEH

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Journal: Journal of Isfahan Medical School Year:2016 | Volume:33 | Issue:368 Page(s): 2461-2468

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Title

COMPARISON OF THE APOPTOSIS INDUCTION EFFECT OF POMEGRANATE PEEL EXTRACT ONHUMAN UMBILICAL VEIN ENDOTHELIAL CELLS AND MELANOMA

Author(s)

DANA NASIM | HAGHJOOY JAVANMARD SHAGHAYEGH | RAFIEE LALEH | Issue Writer Certificate

Keywords

MELANOMAQ2

POMEGRANATE PEEL EXTRACTQ2

APOPTOSISQ3

Abstract

Background: APOPTOSIS defect plays an important role in the formation of tumors and its disruption causes resistance to treatment. The effects of pomegranate on the inhibition of APOPTOSIS and cell proliferation of some cancer types have been demonstrated. The aim of this study was to investigate the effect of black POMEGRANATE PEEL EXTRACT on cell survival, morphology, and APOPTOSIS of MELANOMA cells and human umbilical vein endothelial cells (HUVECs).Methods: The hydroalcoholic extract of black pomegranate pericarp was prepared. Toxicity of MELANOMA and HUVEC was evaluated through MTT assay (3- (4, 5-Dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide) in the experimental group at different concentrations of the extract (10, 100, 200, 300 mg/ml) and dimethyl sulfoxide (DMSO) 0.1% in the control group. In addition, APOPTOSIS was studied using Annexin-V test and flowcytometry. The morphology of the cells was examined under a microscope.Findings: After 48 hours, MELANOMA cell survival significantly decreased in a concentration-dependent manner (P<0.05), but it had no effect on HUVEC proliferation. Exposure of cells to half maximal inhibitory concentration(IC50) at a dose of 77.5 mg/ml led to the induction of early APOPTOSIS (43.05%) and late APOPTOSIS (0.05%) in MELANOMA cells that was significantly increased compared to the control group (P<0.05). In addition, 56.9% of cells were healthy. APOPTOSIS induction was not observed in HUVECs. POMEGRANATE PEEL EXTRACT only in ducedmorphological changes such as cell shrinkage and rounding of cell membrane in MELANOMA cells.Conclusion: POMEGRANATE PEEL EXTRACT induces APOPTOSIS, death, and morphological changes in MELANOMA cells.However, it has no effect on HUVECs. It seems that this extract can be a good candidate for APOPTOSIS inductionin MELANOMA cells as complementary therapy.

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APA: Copy

DANA, NASIM, HAGHJOOY JAVANMARD, SHAGHAYEGH, & RAFIEE, LALEH. (2016). COMPARISON OF THE APOPTOSIS INDUCTION EFFECT OF POMEGRANATE PEEL EXTRACT ONHUMAN UMBILICAL VEIN ENDOTHELIAL CELLS AND MELANOMA. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 33(368), 2461-2468. SID. https://sid.ir/paper/50201/en

Vancouver: Copy

DANA NASIM, HAGHJOOY JAVANMARD SHAGHAYEGH, RAFIEE LALEH. COMPARISON OF THE APOPTOSIS INDUCTION EFFECT OF POMEGRANATE PEEL EXTRACT ONHUMAN UMBILICAL VEIN ENDOTHELIAL CELLS AND MELANOMA. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2016;33(368):2461-2468. Available from: https://sid.ir/paper/50201/en

IEEE: Copy

NASIM DANA, SHAGHAYEGH HAGHJOOY JAVANMARD, and LALEH RAFIEE, “COMPARISON OF THE APOPTOSIS INDUCTION EFFECT OF POMEGRANATE PEEL EXTRACT ONHUMAN UMBILICAL VEIN ENDOTHELIAL CELLS AND MELANOMA,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 33, no. 368, pp. 2461–2468, 2016, [Online]. Available: https://sid.ir/paper/50201/en

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