COMPARISON OF NEURAL STEM CELLS NEUROGENESIS BY USING FLOW CYTOMETRY VERSUS MANUAL COUNTING METHOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AZARI HASAN; GOL MOHAMMADI M.GH.; ESFANDIARI EBRAHIM; MARDANI M.; REYNOLDS B.A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download Full-Text

Persian Verion

View:

1,334

Download:

Cites:

Information Journal Paper

Title

COMPARISON OF NEURAL STEM CELLS NEUROGENESIS BY USING FLOW CYTOMETRY VERSUS MANUAL COUNTING METHOD

Author(s)

Keywords

NEURAL STEM CELLQ2

DIFFERENTIATIONQ2

FLOW CYTOMETRYQ3

NEUROGENESISQ2

Abstract

Background: Finding factors that can increase NEUROGENESIS are of great importance. To find these factors, it seems that NEURAL STEM CELLs culture is an ideal method. To analyze the effect of different factors in a limited period of time and with the least cost, finding an easier and more efficient method than normal manual counting method is needed. The aim of this study was using FLOW CYTOMETRY as an alternative method to evaluate NEURAL STEM CELLs NEUROGENESIS.Methods: NEURAL STEM CELLs from E14 mouse brain have been differentiated in a one- step and a two -step methods. After performing immunohistochemistry for neuronal and astrocytic markers, manual and FLOW CYTOMETRY methods have been compared in determining the percentage of neurons and astrocytes. Then, the percentage of neurons and astrocytes generated in two different DIFFERENTIATION methods has been compared using FLOW CYTOMETRY.Findings: Our findings showed that there wasn't any statistical difference between manual and FLOW CYTOMETRY methods in determining the percentage of neurons and astrocytes. Comparing DIFFERENTIATION methods by FLOW CYTOMETRY, showed that the percentage of both neurons and astrocytes were significantly different in these two methods (p<0.001).Conclusion: FLOW CYTOMETRY is a simple and reliable method that can replace manual counting method to evaluate NEUROGENESIS of the NEURAL STEM CELLs. This method would be very useful especially when a high content screening of different factors and compounds is needed.

Cites

No record.

References

No record.

Cite

APA: Copy

AZARI, HASAN, GOL MOHAMMADI, M.GH., ESFANDIARI, EBRAHIM, MARDANI, M., & REYNOLDS, B.A.. (2007). COMPARISON OF NEURAL STEM CELLS NEUROGENESIS BY USING FLOW CYTOMETRY VERSUS MANUAL COUNTING METHOD. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 25(86), 9-18. SID. https://sid.ir/paper/50819/en

Vancouver: Copy

AZARI HASAN, GOL MOHAMMADI M.GH., ESFANDIARI EBRAHIM, MARDANI M., REYNOLDS B.A.. COMPARISON OF NEURAL STEM CELLS NEUROGENESIS BY USING FLOW CYTOMETRY VERSUS MANUAL COUNTING METHOD. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2007;25(86):9-18. Available from: https://sid.ir/paper/50819/en

IEEE: Copy

HASAN AZARI, M.GH. GOL MOHAMMADI, EBRAHIM ESFANDIARI, M. MARDANI, and B.A. REYNOLDS, “COMPARISON OF NEURAL STEM CELLS NEUROGENESIS BY USING FLOW CYTOMETRY VERSUS MANUAL COUNTING METHOD,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 25, no. 86, pp. 9–18, 2007, [Online]. Available: https://sid.ir/paper/50819/en

Related Journal Papers