EFFECT OF MEDIUM IN PROPERTIES AND ACTIVITY OF ACP IN THE INFECTIVE FORM OF LEISHMANIA MAJOR PROMASTIGOTES

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NAVABI AMIR; SOLEIMANIFARD SIMINDOKHT

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Journal: Journal of Isfahan Medical School Year:2014 | Volume:32 | Issue:295 Page(s): 1-9

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Title

EFFECT OF MEDIUM IN PROPERTIES AND ACTIVITY OF ACP IN THE INFECTIVE FORM OF LEISHMANIA MAJOR PROMASTIGOTES

Author(s)

NAVABI AMIR | SOLEIMANIFARD SIMINDOKHT | Issue Writer Certificate

Keywords

LEISHMANIA MAJORQ2

MEDIA CULTUREQ3

ACID PHOSPHATASEQ3

VIRULENCEQ3

Abstract

Background: The wet cutaneus leishmaniasis caused by Leis hmania major infection is one of the common skin disease in many parts of the world. Reports indicate that some properties of Leis hmania such as time of infection is affected of medium. With respect to use to artificial medium in the majority of the studies on Leismania and the role of ACID PHOSPHATASE in the pathogenesis of promastigotes, in this research, were studied effects of medium in properties of ACP and time of infecting in L.major promastigotes.Methods: The present study is a cross sectional study. To culture promastigotes, L.major (MRHO/IR/75/ER) from previously infected Balb/c mice was transferred to NNN and RPMI1640 medium. Growth curve was generated and stationary stages were divided. Frozen promastigotes of each medium with sodium acetate and Triton-X-100 were homogenize and ACP was measured by calorimetric assay.Findings: Stationary parasites were collected in NNN and RPMI-1640 medium in seventh and fifth day respectively. The rate of ACP activity was determined, 1.02±0.08 in NNN and 1.8±0.01 in RPMI- 1640. Also Km and Vmax of this enzyme was 105.26±1.11 mM and 98.37±1.48 mM/min/mg protein in NNN and 106.39±1.14 mM and 98.04±0.96 mM/min/mg protein in RPMI1640.Conclusion: Despite the infecting time in promastigotes, rate of agglutination, rate of kinase synthesis and virulent are different in two medium, it seems there are no significant differences in properties of ACID PHOSPHATASE in stationary promastigotes in two medium.

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APA: Copy

NAVABI, AMIR, & SOLEIMANIFARD, SIMINDOKHT. (2014). EFFECT OF MEDIUM IN PROPERTIES AND ACTIVITY OF ACP IN THE INFECTIVE FORM OF LEISHMANIA MAJOR PROMASTIGOTES. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 32(295), 1-9. SID. https://sid.ir/paper/50950/en

Vancouver: Copy

NAVABI AMIR, SOLEIMANIFARD SIMINDOKHT. EFFECT OF MEDIUM IN PROPERTIES AND ACTIVITY OF ACP IN THE INFECTIVE FORM OF LEISHMANIA MAJOR PROMASTIGOTES. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2014;32(295):1-9. Available from: https://sid.ir/paper/50950/en

IEEE: Copy

AMIR NAVABI, and SIMINDOKHT SOLEIMANIFARD, “EFFECT OF MEDIUM IN PROPERTIES AND ACTIVITY OF ACP IN THE INFECTIVE FORM OF LEISHMANIA MAJOR PROMASTIGOTES,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 32, no. 295, pp. 1–9, 2014, [Online]. Available: https://sid.ir/paper/50950/en

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