EFFECT OF DEXAMETHASONE ON VIABILITY OF CARTILAGE GRAFTS IN PRESERVING MEDIAS

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KHORASANI GHASEMALI; MIRI ROZA; GHANBARZADE KOROUSH; FARZAD HOOMAN; FARHADI HAMED

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Journal: MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES Year:2016 | Volume:59 | Issue:3 Page(s): 155-162

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Title

EFFECT OF DEXAMETHASONE ON VIABILITY OF CARTILAGE GRAFTS IN PRESERVING MEDIAS

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Keywords

CHONDROCYTES VIABILITYQ1

ETHANOL AND DEXAMETHASONE MEDIAQ1

ETHANOL MEDIAQ1

Abstract

Introduction: cartilage grafts have a fundamental role in reconstructive and aesthetic surgeries. Preservation of these grafts in suitable Medias can provide them for revision surgeries. Previous studies have shown that ethanol is a suitable media to preserve garfts. In this study, we assess the effect of Dexamethasone addition on viability of cartilage grafts and documented this with histopathological studies.Methods: fifteen male New zealand rabbits that were purchased by Pasteur institute, Karaj, Iran, were evaluated in this study from March 2014 to July 2014. Cartilage grafts of rabbit ear with preservation of perichondrium preserved in 70 % Ethanol with and without adding of 10 microgram of dexamethasone stored in 4 c. After 45 and 90 days, these samples evaluated by histopahtological H& E staining and viability of chondrocytes determined by evidence of karyorrhexis and kariolysis in each sample.Results: viability of chondrocytes in ethanol 70 was 77.29±9.23 and viability in ETHANOL AND DEXAMETHASONE MEDIA was 89.42±10.71after 45 days (p<0.04). Viability of chondrocytes in ethanol 70 % was 71.79±9.30 and viability in ethanol and dexamethasone was 83.50±10.73 after 90 days (p<0.06).Conclusion: addition of dexamethasone in preserving medias of cartilage grafts can increase the viability of chondrocytes and viable chondrocytes have a fundamental role in graft stability and make them usable for revision surgeries.

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APA: Copy

KHORASANI, GHASEMALI, MIRI, ROZA, GHANBARZADE, KOROUSH, FARZAD, HOOMAN, & FARHADI, HAMED. (2016). EFFECT OF DEXAMETHASONE ON VIABILITY OF CARTILAGE GRAFTS IN PRESERVING MEDIAS. MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES, 59(3), 155-162. SID. https://sid.ir/paper/51367/en

Vancouver: Copy

KHORASANI GHASEMALI, MIRI ROZA, GHANBARZADE KOROUSH, FARZAD HOOMAN, FARHADI HAMED. EFFECT OF DEXAMETHASONE ON VIABILITY OF CARTILAGE GRAFTS IN PRESERVING MEDIAS. MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;59(3):155-162. Available from: https://sid.ir/paper/51367/en

IEEE: Copy

GHASEMALI KHORASANI, ROZA MIRI, KOROUSH GHANBARZADE, HOOMAN FARZAD, and HAMED FARHADI, “EFFECT OF DEXAMETHASONE ON VIABILITY OF CARTILAGE GRAFTS IN PRESERVING MEDIAS,” MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES, vol. 59, no. 3, pp. 155–162, 2016, [Online]. Available: https://sid.ir/paper/51367/en

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