EVALUATION OF SYSTEMIC AND MUCOSAL IMMUNE RESPONSES FOLLOWING THE NASAL IMMUNIZATION BY VARIOUS SIZES OF LIPOSOMES ENCAPSULATED WITH TETANUS TOXOID

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TAFAGHODI M.; JAFARI M.R.; NIKOUZADEH A.

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Journal: Iranian Journal of Basic Medical Sciences Year:2007 | Volume:9 | Issue:4 (32) Page(s): 223-230

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Title

EVALUATION OF SYSTEMIC AND MUCOSAL IMMUNE RESPONSES FOLLOWING THE NASAL IMMUNIZATION BY VARIOUS SIZES OF LIPOSOMES ENCAPSULATED WITH TETANUS TOXOID

Author(s)

TAFAGHODI M. | JAFARI M.R. | NIKOUZADEH A. | Issue Writer Certificate

Keywords

LIPOSOMESQ3

IMMUNIZATIONQ3

TETANUS TOXOIDQ4

Abstract

ObjectiveLIPOSOMES have been identified as effective immunological adjuvants and have potential for the intranasal and oral delivery of protein antigens. The physicochemical properties of LIPOSOMES including the liposome size can influence their utility as a delivery system and vaccine adjuvant. Materials and MethodsFollowing nasal administration of LIPOSOMES, the effect of various sizes of extruded LIPOSOMES encapsulated with TETANUS TOXOID (TT), on the induction of systemic and mucosal immune responses were studied. LIPOSOMES containing TT were prepared by dehydration-rehydration method (DRV) from phosphatidylcholine (PC) and cholestrol. The 1000,400 and 100 nm sized LIPOSOMES were prepared from DRV LIPOSOMES by extrusion method. Animals were immunized three times with liposomal or solution of 40 Lf TT. The serum IgG titers and nasal lavage sIgA titers were determined by ELISA method.ResultsThe volume mean diameter of DRVs was 2.2 mm. Encapsulation efficiency of TT in LIPOSOMES ranged betvyeen64.3 -74% (40 LfTT/ 200 ml liposome suspension). Encapsulated TT kept its intact structure, as shown by SDS-PAGE method. The highest serum IgG titers were observed in groups immunized with solution formulations (p< 0.05). However the highest mucosal sIgA titers were achieved by 100nm sized LIPOSOMES. Among the liposomal formulations, the highest serum IgG titers were observed in DRV group (p< 0.001). It is concluded that the mucosal and systemic immune responses are differently affected by liposome size. The mucosal immune responses were increased by 100 nm LIPOSOMES, but the systemic immune responses were suppressed.ConclusionAfter nasal IMMUNIZATION with liposomal TT, however, a relation between the liposome size and mucosal or systemic immune responses could not be indicated.

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APA: Copy

TAFAGHODI, M., JAFARI, M.R., & NIKOUZADEH, A.. (2007). EVALUATION OF SYSTEMIC AND MUCOSAL IMMUNE RESPONSES FOLLOWING THE NASAL IMMUNIZATION BY VARIOUS SIZES OF LIPOSOMES ENCAPSULATED WITH TETANUS TOXOID. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 9(4 (32)), 223-230. SID. https://sid.ir/paper/52391/en

Vancouver: Copy

TAFAGHODI M., JAFARI M.R., NIKOUZADEH A.. EVALUATION OF SYSTEMIC AND MUCOSAL IMMUNE RESPONSES FOLLOWING THE NASAL IMMUNIZATION BY VARIOUS SIZES OF LIPOSOMES ENCAPSULATED WITH TETANUS TOXOID. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2007;9(4 (32)):223-230. Available from: https://sid.ir/paper/52391/en

IEEE: Copy

M. TAFAGHODI, M.R. JAFARI, and A. NIKOUZADEH, “EVALUATION OF SYSTEMIC AND MUCOSAL IMMUNE RESPONSES FOLLOWING THE NASAL IMMUNIZATION BY VARIOUS SIZES OF LIPOSOMES ENCAPSULATED WITH TETANUS TOXOID,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 9, no. 4 (32), pp. 223–230, 2007, [Online]. Available: https://sid.ir/paper/52391/en

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