ISOLATION OF ZYMODEME I OF ENTAMOEBA DISPAR USING ISOENZYME ANALYSIS METHOD IN IRAN

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HAGHIGHI A.; REZAEIAN M.

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Paper Information

Journal: Iranian Journal of Basic Medical Sciences Year:2006 | Volume:9 | Issue:1 (29) Page(s): 19-23

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Information Journal Paper

Title

ISOLATION OF ZYMODEME I OF ENTAMOEBA DISPAR USING ISOENZYME ANALYSIS METHOD IN IRAN

Author(s)

HAGHIGHI A. | REZAEIAN M. | Issue Writer Certificate

Keywords

ISOENZYMEQ3

ZYMODEMEQ3

ENTAMOEBA DISPARQ4

AXENICQ3

XENICQ3

Abstract

Objective: Differential diagnosis of Entamoeba histolytica and ENTAMOEBA DISPAR, which are two morphologically identical species of amoebae is essential both for the treatment decision and public health aspects. The electrophoretic patterns of hexokinase and phosphoglucomutase have been widely used to distinguish E. histolytica from E. dispar and ISOENZYME analysis is still a gold standard method. This study was done to determin the ISOENZYME patterns of E. histolytica and E. dispar in Iran. Materials and Methods: In this study electrophoretic patterns of two Iranian Entamoeba isolates and four standard E. histolytica and E. dispar were analyzed by using four enzymes: Glucose phosphate isomerase (GPI), phosphoglucomutase (PGM), L-malate : NADP+ oxido-reductase (ME) and hexokinase (HK). Results: Both Iranian isolates gave ZYMODEME I pattern of E. dispar. After axenization of the isolates in YIGADHA-S medium, the electerophoretic patterns of ZYMODEME I remained stable without any changes. Conclusion: Our study confirmed the existence of ZYMODEME I of E. dispar in Iran. The result of this research and another experiment on 8 isolates led us to declare that ZYMODEME I might be the dominant pattern of E. dispar ZYMODEMEs in Iran.

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References

DIFFERENTIATION OF ENTAMEBA HISTOLYTICA AND ENTAMOEBA DISPAR BY PCR-RFLP (ISOLATES OF TEHRAN, IRAN)

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APA: Copy

HAGHIGHI, A., & REZAEIAN, M.. (2006). ISOLATION OF ZYMODEME I OF ENTAMOEBA DISPAR USING ISOENZYME ANALYSIS METHOD IN IRAN. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 9(1 (29)), 19-23. SID. https://sid.ir/paper/52483/en

Vancouver: Copy

HAGHIGHI A., REZAEIAN M.. ISOLATION OF ZYMODEME I OF ENTAMOEBA DISPAR USING ISOENZYME ANALYSIS METHOD IN IRAN. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2006;9(1 (29)):19-23. Available from: https://sid.ir/paper/52483/en

IEEE: Copy

A. HAGHIGHI, and M. REZAEIAN, “ISOLATION OF ZYMODEME I OF ENTAMOEBA DISPAR USING ISOENZYME ANALYSIS METHOD IN IRAN,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 9, no. 1 (29), pp. 19–23, 2006, [Online]. Available: https://sid.ir/paper/52483/en

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