THE EFFECT OF DIFFERENT CONCENTRATIONS OF CULTURE MEDIUM ON TOXIN PRODUCTION AND EXTINCTION COEFFICIENT OF BOTULINUM NEUROTOXIN TYPE A

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ATAEI R.A.; BEHZADIANNEZHAD GH.

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2002 | Volume:7 | Issue:17 Page(s): 70-75

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Title

THE EFFECT OF DIFFERENT CONCENTRATIONS OF CULTURE MEDIUM ON TOXIN PRODUCTION AND EXTINCTION COEFFICIENT OF BOTULINUM NEUROTOXIN TYPE A

Author(s)

ATAEI R.A. | BEHZADIANNEZHAD GH. | Issue Writer Certificate

Keywords

NEUROTOXIN TYPE AQ4

CLOSTRIDIUM BOTULINUMQ4

EXTINCTION OF COEFFICIENTQ4

Abstract

Clostridium botulinum Type A Neurotoxin is one of the 7 recognized types that is available in clinical use. The reference standard criteria for medical uses of toxin is necessary. It should be based on its chemical and physical properties, such as: extinction coefficient as an equal 1.65 and a 260/278 nm absorbance ratio of <0.55 to quantitatively measure the toxin. This study was conducted to examine the effect of different ingredient culture media on growth, protein production, extinction coefficient and 260/278 nm absorbance ratio of Neurotoxin Clostridium type A. The different concentration of culture media was made, each if them were spectrophotometry scanned in 200 nm. Then inoculated with preculture and 300-anearobicly incubation was carried out. After incubation period, growth and protein production was assayed- Nerutoxin production was tested in albino mice by inducing paralysis through i.p. injection. In addition to toxicity assay, the production and extraction of toxin sheme were monitored by 260/278 nm absorbance ratio and EXTINCTION OF COEFFICIENT was calculated respectively. The results showed that, a change of ingredient culture media, could be produced toxin with appropriate extinction coefficient and ratio of absorbance.

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APA: Copy

ATAEI, R.A., & BEHZADIANNEZHAD, GH.. (2002). THE EFFECT OF DIFFERENT CONCENTRATIONS OF CULTURE MEDIUM ON TOXIN PRODUCTION AND EXTINCTION COEFFICIENT OF BOTULINUM NEUROTOXIN TYPE A. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 7(17), 70-75. SID. https://sid.ir/paper/52785/en

Vancouver: Copy

ATAEI R.A., BEHZADIANNEZHAD GH.. THE EFFECT OF DIFFERENT CONCENTRATIONS OF CULTURE MEDIUM ON TOXIN PRODUCTION AND EXTINCTION COEFFICIENT OF BOTULINUM NEUROTOXIN TYPE A. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2002;7(17):70-75. Available from: https://sid.ir/paper/52785/en

IEEE: Copy

R.A. ATAEI, and GH. BEHZADIANNEZHAD, “THE EFFECT OF DIFFERENT CONCENTRATIONS OF CULTURE MEDIUM ON TOXIN PRODUCTION AND EXTINCTION COEFFICIENT OF BOTULINUM NEUROTOXIN TYPE A,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 7, no. 17, pp. 70–75, 2002, [Online]. Available: https://sid.ir/paper/52785/en

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