VARIOUS MORPHOLOGIC SHAPES OF LEISHMANIA MAJOR AMASTIGOTES WITH LOW GENETIC STRUCTURE AMONG PEOPLE AFFLICTED WITH ZOONOTIC CUTANEOUS LEISHMANIASIS IN KHUZESTAN PROVINCE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SOLTAN SEYED SHOJA; SPOTIN ADEL; EBRAHIMI SAHAR; Alaeenovin Elnaz; BORDBAR ALI; PARVIZI PARVIZ

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2016 | Volume:21 | Issue:74 Page(s): 61-74

Download Full-Text

Persian Verion

View:

664

Download:

Cites:

Information Journal Paper

Title

VARIOUS MORPHOLOGIC SHAPES OF LEISHMANIA MAJOR AMASTIGOTES WITH LOW GENETIC STRUCTURE AMONG PEOPLE AFFLICTED WITH ZOONOTIC CUTANEOUS LEISHMANIASIS IN KHUZESTAN PROVINCE

Author(s)

Keywords

LEISHMANIA MAJORQ2

CLINICAL MANIFESTATIONSQ2

AMASTIGOTE SHAPESQ1

GENETIC VARIATIONSQ1

KHUZESTANQ3

Abstract

Background and objective: Tegumentary leishmaniasis has been implicated in many types of different phenotypes due to diverse genetic extent with polymorphic AMASTIGOTE SHAPES. Molecular diversity and morphological changes of LEISHMANIA MAJOR (L. major) have turned into a controversial issue among researchers. This investigation revealed the features of amastigote polymorphic shapes in CLINICAL MANIFESTATIONS of suspected patients to zoonotic cutaneous leishmaniasis (ZCL) in the direction of genetic variation in KHUZESTAN province.Materials and method: Of 315 suspected patients, sampling, smear preparation and staining were carried out during 2012-2014. After DNA extraction, the PCR products were digested with BsuRI endonuclease enzyme in the process of RFLP by targeting ITS-rDNA, Cyt b and Microsatellite genes.Results: L. major and L. tropica were the parasites identified in patients having regular amastigotes' shapes (oval or round) with a size of 2-4 μm in each of dry, wet and classical mixed lesions. Whereas, irregular shapes (spindle, pear or cigarette) were observed in non-classical wet lesions with more than 4 μm. Sequencing of ITS-rDNA and Cyt b genes of L. major demonstrated low molecular variation (P >0.05) except for one common haplotype (GenBank access no. EF413075).Conclusion: On the basis of present findings, there is no significant correlation between phenotypic and genotypic features of L. major isolates. This study is considered as the first exhaustive report to incriminate morphometric shapes of L. major amastigotes concerning their relevance with various clinical appearances and genotypic characteristics. LEISHMANIA MAJOR, CLINICAL MANIFESTATIONS, AMASTIGOTE SHAPES, GENETIC VARIATIONS, KHUZESTAN

Cites

No record.

References

Cite

APA: Copy

SOLTAN, SEYED SHOJA, SPOTIN, ADEL, EBRAHIMI, SAHAR, Alaeenovin, Elnaz, BORDBAR, ALI, & PARVIZI, PARVIZ. (2016). VARIOUS MORPHOLOGIC SHAPES OF LEISHMANIA MAJOR AMASTIGOTES WITH LOW GENETIC STRUCTURE AMONG PEOPLE AFFLICTED WITH ZOONOTIC CUTANEOUS LEISHMANIASIS IN KHUZESTAN PROVINCE. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 21(74), 61-74. SID. https://sid.ir/paper/52867/en

Vancouver: Copy

SOLTAN SEYED SHOJA, SPOTIN ADEL, EBRAHIMI SAHAR, Alaeenovin Elnaz, BORDBAR ALI, PARVIZI PARVIZ. VARIOUS MORPHOLOGIC SHAPES OF LEISHMANIA MAJOR AMASTIGOTES WITH LOW GENETIC STRUCTURE AMONG PEOPLE AFFLICTED WITH ZOONOTIC CUTANEOUS LEISHMANIASIS IN KHUZESTAN PROVINCE. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2016;21(74):61-74. Available from: https://sid.ir/paper/52867/en

IEEE: Copy

SEYED SHOJA SOLTAN, ADEL SPOTIN, SAHAR EBRAHIMI, Elnaz Alaeenovin, ALI BORDBAR, and PARVIZ PARVIZI, “VARIOUS MORPHOLOGIC SHAPES OF LEISHMANIA MAJOR AMASTIGOTES WITH LOW GENETIC STRUCTURE AMONG PEOPLE AFFLICTED WITH ZOONOTIC CUTANEOUS LEISHMANIASIS IN KHUZESTAN PROVINCE,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 21, no. 74, pp. 61–74, 2016, [Online]. Available: https://sid.ir/paper/52867/en

Related Journal Papers