EVALUATION OF IMMUNOGENICITY OF TRANSGENIC LEISHMANIA MAJOR IN BALB/C MICE

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DOUSTI A.; DAVOUDI N.

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2006 | Volume:11 | Issue:34 Page(s): 19-22

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Title

EVALUATION OF IMMUNOGENICITY OF TRANSGENIC LEISHMANIA MAJOR IN BALB/C MICE

Author(s)

DOUSTI A. | DAVOUDI N. | Issue Writer Certificate

Keywords

LEISHMANIA MAJORQ2

TRANSGENICQ3

VACCINEQ3

Abstract

Background and objective: Since once infection with Leishmania gives raise permanent immunity. The development of an affordable VACCINE is considered the only cost-effective means to control leishmaniasis.Material and methods: In present study TRANSGENIC strain of L.major which express two suicide genes; thymidine kinase gene of Herpes Simplex Virus type one (HSV-tk) and cytosine deaminase gene of Saccharomyces cervisiea (Se-cd) in its genome have been used for vaccination of mice. Susceptible BALB/c mice were infected by TRANSGENIC L.major and treated for 2 consecutive weeks with ganciclovir and 5-fluorocytosine both drug together, after one week at the initial infection.Results: Treatment with these drugs at varying groups after infection showed different effects on the outcome of disease. The goal of this study was, assaying the immunogenesity obtain by vaccination of mice with TRANSGENIC L.major. Start of drug administration on day 7 after initial infection showed eradicating of parasites in BALB/c mice. Challenge with wild type L.major in vaccinated and no vaccinated mice showed different results. After challenge in vaccinated BALB/c mice, it was attenuated and delayed disease progression, which was due partial immunity. The ELISA cytokine assay in this group showed high increase of IFN -γ and low IL-4 in compare with control mice (no vaccinated mice).Conclusion: This result confirms high level of immunity against Leishmaniasis in vaccinated BALB/c mice with TRANSGENIC L.major. The inducible expression of suicide gene products represents a valuable tool for the development of safe and effective VACCINEs.

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APA: Copy

DOUSTI, A., & DAVOUDI, N.. (2006). EVALUATION OF IMMUNOGENICITY OF TRANSGENIC LEISHMANIA MAJOR IN BALB/C MICE. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 11(34), 19-22. SID. https://sid.ir/paper/52878/en

Vancouver: Copy

DOUSTI A., DAVOUDI N.. EVALUATION OF IMMUNOGENICITY OF TRANSGENIC LEISHMANIA MAJOR IN BALB/C MICE. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2006;11(34):19-22. Available from: https://sid.ir/paper/52878/en

IEEE: Copy

A. DOUSTI, and N. DAVOUDI, “EVALUATION OF IMMUNOGENICITY OF TRANSGENIC LEISHMANIA MAJOR IN BALB/C MICE,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 11, no. 34, pp. 19–22, 2006, [Online]. Available: https://sid.ir/paper/52878/en

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