LARGE-SCALE PRODUCTION OF ANTI-RH IMMUNE GLOBULIN BY ION EXCHANGE CHROMATOGRAPHY

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VARASTEH A.R.; HASHEMI M.; FAZLI BAZAZ B.S.; GHAZAVI A.; BAHRAMI A.A.; OMIDI A.; ORAFAEI H.; HASANI A.; TABATABAEI A.; BAHRAMI A.

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Journal: Iranian Journal of Basic Medical Sciences Year:2001 | Volume:4 | Issue:3 (11) Page(s): 16-21

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Title

LARGE-SCALE PRODUCTION OF ANTI-RH IMMUNE GLOBULIN BY ION EXCHANGE CHROMATOGRAPHY

Author(s)

Keywords

ANTI-D

ION EXCHANGE CHROMATOGRAPHY

PURIFICATION

Abstract

Most immunoglobulin preparations are produced by methods based on the Cohn ethanol precipitation technique and recommended for intramuscular administration only. In this research, large-scale production of ANTI-D IgG as an intravenous preparation by ION EXCHANGE CHROMATOGRAPHY is described. The method including: absorption of plasma proteins onto DEAE-Sephadex A50 except ANTI-D IgG, washing of the gel and elution of the ANTI-D IgG with 0.025 M phosphate buffer at pH 7.5, followed by eluted ANTI-D IgG concentration on a pellicon ultrafiltration system, addition of protein stabilizers, sterile filtration, and freeze-drying of the final product. Analysis of the product indicated that it consisted principally of the IgG monomer with traces of fragmentation or aggregation, and anticomplemantry activity was Il1significant.Amount of IgA and IgM were not higher than 10 µ.g/ml. Stability data of ANTI-D preparation at 37°C for 4 weeks showed no considerable changes in antibody potency and safety, pyrogen and sterility tests meet the requirements set by USP. ANTI-D prepared has low anticomplemantary activity and IgA and hence intravenous tolerate.

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APA: Copy

VARASTEH, A.R., HASHEMI, M., FAZLI BAZAZ, B.S., GHAZAVI, A., BAHRAMI, A.A., OMIDI, A., ORAFAEI, H., HASANI, A., TABATABAEI, A., & BAHRAMI, A.. (2001). LARGE-SCALE PRODUCTION OF ANTI-RH IMMUNE GLOBULIN BY ION EXCHANGE CHROMATOGRAPHY. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 4(3 (11)), 16-21. SID. https://sid.ir/paper/528813/en

Vancouver: Copy

VARASTEH A.R., HASHEMI M., FAZLI BAZAZ B.S., GHAZAVI A., BAHRAMI A.A., OMIDI A., ORAFAEI H., HASANI A., TABATABAEI A., BAHRAMI A.. LARGE-SCALE PRODUCTION OF ANTI-RH IMMUNE GLOBULIN BY ION EXCHANGE CHROMATOGRAPHY. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2001;4(3 (11)):16-21. Available from: https://sid.ir/paper/528813/en

IEEE: Copy

A.R. VARASTEH, M. HASHEMI, B.S. FAZLI BAZAZ, A. GHAZAVI, A.A. BAHRAMI, A. OMIDI, H. ORAFAEI, A. HASANI, A. TABATABAEI, and A. BAHRAMI, “LARGE-SCALE PRODUCTION OF ANTI-RH IMMUNE GLOBULIN BY ION EXCHANGE CHROMATOGRAPHY,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 4, no. 3 (11), pp. 16–21, 2001, [Online]. Available: https://sid.ir/paper/528813/en

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