THE CO-CULTURE WITH VERO CELLS AND THE DEVELOPMENT OF TWO CELL MOUSE EMBRYOS AFTER VITRIFICATION

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MOVAHEDIN M.; REZAZADEH VALOUJERDI M.; HOSSEINI A.

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Journal Paper

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Journal: Cell Journal (Yakhteh) Year:1999 | Volume:1 | Issue:3 Page(s): 0-0

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Title

THE CO-CULTURE WITH VERO CELLS AND THE DEVELOPMENT OF TWO CELL MOUSE EMBRYOS AFTER VITRIFICATION

Author(s)

MOVAHEDIN M. | REZAZADEH VALOUJERDI M. | HOSSEINI A. | Issue Writer Certificate

Keywords

VITRIFICATION

ETHYLENE GLYCOL

CO-CULTURE

TWO CELL MOUSE EMBRYOS.

Abstract

Introduction: The Mouse embryos can successfully be vitrified using ETHYLENE GLYCOL as cryoprotectant, however their development differs significantly from the non-vitrified embryos. The ability of their development can be improved when they CO-CULTURE with somatic cells. In the present study, the effects of co-culturing with Vero cells on the development of vitrified TWO CELL MOUSE EMBRYOS.were studied. Materials and Methods: Two cell embryos were flushed from the excised uteri of superovulated mice. Morphologically normal embryos were vitrified using 10% ETHYLENE GLYCOL solution (EFS10), and thawed rapidly using 0.5 M sucrose solution. The survived embryos were divided to two grops: one group cultured on RPMI and the another one CO-CULTUREd with Vero cells. Control embryos was considered for each experimental groups. Results: The survival rate was 83%. The developmental rate of embryos which could reach to morula stage for the exp. group 1 and 2 were 52% and 69% respectively which the difference between the exp. groups was significant (P<0.001). Mean while 48% of the exp. group 1 could form blastocysts, accordingly the second group showed 60% and the difference was significant (P<0.001). The difference between the two exp. groups which could reach to hatching stage was not significant. Conclusion: The results suggest that the CO-CULTURE with Vero cells can improve the development of vitrified TWO CELL MOUSE EMBRYOS.

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APA: Copy

MOVAHEDIN, M., REZAZADEH VALOUJERDI, M., & HOSSEINI, A.. (1999). THE CO-CULTURE WITH VERO CELLS AND THE DEVELOPMENT OF TWO CELL MOUSE EMBRYOS AFTER VITRIFICATION . CELL JOURNAL (YAKHTEH), 1(3), 0-0. SID. https://sid.ir/paper/529020/en

Vancouver: Copy

MOVAHEDIN M., REZAZADEH VALOUJERDI M., HOSSEINI A.. THE CO-CULTURE WITH VERO CELLS AND THE DEVELOPMENT OF TWO CELL MOUSE EMBRYOS AFTER VITRIFICATION . CELL JOURNAL (YAKHTEH)[Internet]. 1999;1(3):0-0. Available from: https://sid.ir/paper/529020/en

IEEE: Copy

M. MOVAHEDIN, M. REZAZADEH VALOUJERDI, and A. HOSSEINI, “THE CO-CULTURE WITH VERO CELLS AND THE DEVELOPMENT OF TWO CELL MOUSE EMBRYOS AFTER VITRIFICATION ,” CELL JOURNAL (YAKHTEH), vol. 1, no. 3, pp. 0–0, 1999, [Online]. Available: https://sid.ir/paper/529020/en

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