VITRIFICATION OF SINGLE HUMAN SPERMATOZOON IN 10% PVP USING ICSI MICRO-TOOL AS THE VESSEL

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SADRI H.A.

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Journal Paper

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Journal: Cell Journal (Yakhteh) Year:2005 | Volume:7 | Issue:SUPPLEMENT 1 Page(s): 29-30

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Title

VITRIFICATION OF SINGLE HUMAN SPERMATOZOON IN 10% PVP USING ICSI MICRO-TOOL AS THE VESSEL

Author(s)

SADRI H.A.

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Abstract

The purpose of this study was to determine the feasibility of utilizing ICSI micro-tool as the vessel for rapid cooling and post-thaw recovery of single human spermatozoa in the presence of 10% PVP as the cryoprotectant. The procedures of loading, sealing, and recovery were carried out using a micromanipulator.
Motile spermatozoa from 3 healthy specimens were chosen randomly for single cell cryopreservation, and their fertilizing ability was compared to that of the some specimens while cryopreserved conventionally. A single spermatozoon was loaded in the tip of the micro-tool. The tip was then sealed in a drying micro-drop of PVP, uncovered with mineral oil.
The sealed micro-tool was then removed from the micromanipulator, and each was fixed in a separate cryo-vial before being plunged into LNz. After thawing at room temperature, the seal was dissolved in the injection medium shortly after being exposed to the drop. The injection was carried out using the same micro-tool.
One-day old human eggs were used to assess the fertilizing ability. A total of 21 individually cryopreserved spermatozoa were injected into spare eggs, of which 16 (76.1%) became fertilized; it was comparable to fertilizing ability of the conventionally cryopreserved specimens.
The fragility of the glass micro-capillary was not increased, partly due to small diameter of the vessel which allowed for thermal equilibration to occur almost instantly.

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APA: Copy

SADRI, H.A.. (2005). VITRIFICATION OF SINGLE HUMAN SPERMATOZOON IN 10% PVP USING ICSI MICRO-TOOL AS THE VESSEL. CELL JOURNAL (YAKHTEH), 7(SUPPLEMENT 1), 29-30. SID. https://sid.ir/paper/530087/en

Vancouver: Copy

SADRI H.A.. VITRIFICATION OF SINGLE HUMAN SPERMATOZOON IN 10% PVP USING ICSI MICRO-TOOL AS THE VESSEL. CELL JOURNAL (YAKHTEH)[Internet]. 2005;7(SUPPLEMENT 1):29-30. Available from: https://sid.ir/paper/530087/en

IEEE: Copy

H.A. SADRI, “VITRIFICATION OF SINGLE HUMAN SPERMATOZOON IN 10% PVP USING ICSI MICRO-TOOL AS THE VESSEL,” CELL JOURNAL (YAKHTEH), vol. 7, no. SUPPLEMENT 1, pp. 29–30, 2005, [Online]. Available: https://sid.ir/paper/530087/en

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