APPLICATION OF HIGHT PERFORMANCE LIQUID CHROMATOGRAPHY WITH SPECTROFLUORIMETRIC DETECTION FOR DETERMINATION OF HUMAN MILK RETINOL

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FALAHAT PISHEH H.R.; GHARAVI NOORI A.

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Journal Paper

Paper Information

Journal: Tehran University Medical Journal Year:2001 | Volume:59 | Issue:6 Page(s): 86-91

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Information Journal Paper

Title

APPLICATION OF HIGHT PERFORMANCE LIQUID CHROMATOGRAPHY WITH SPECTROFLUORIMETRIC DETECTION FOR DETERMINATION OF HUMAN MILK RETINOL

Author(s)

FALAHAT PISHEH H.R. | GHARAVI NOORI A. | Issue Writer Certificate

Keywords

DETERMINATION

ALL-TRANS RETINOL

HPLC

SPECROFLUOROMEMETRIC DETECTION

Abstract

World health organization has introduced concentration of vitamin A in breast milk as a proper indicator for characterization of vitamin A deficiency in lactating mothers and their infants (<6 mo). A normal phase high performance liquid chromatography with spectrofluorometric detection as a specific and sensitive detector was used for characterization of all-trans retional from 13-Cis retinal. The method was sensitive (0.009 ppm) and accurate (1±0.054 µ mol/l) and had a good recovery percentage (99.36%). This method was more better than before methods and was compatible with the other useful methods. Effect of freezing and defreezing on ALL-TRANS RETINOL content of milk sample was investigated. The result was interesting, whatever ALL-TRANS RETINOL content was bigger, it’s destroying was bigger too. Because of that reseon, day today reproducibility wasn’t good. Standard of retinol was 95% all-trans and pure so we should use this method because two retinol isomers wasn’t separated from each other by reversed phase chromatography and UV detection.

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APA: Copy

FALAHAT PISHEH, H.R., & GHARAVI NOORI, A.. (2001). APPLICATION OF HIGHT PERFORMANCE LIQUID CHROMATOGRAPHY WITH SPECTROFLUORIMETRIC DETECTION FOR DETERMINATION OF HUMAN MILK RETINOL. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 59(6), 86-91. SID. https://sid.ir/paper/531723/en

Vancouver: Copy

FALAHAT PISHEH H.R., GHARAVI NOORI A.. APPLICATION OF HIGHT PERFORMANCE LIQUID CHROMATOGRAPHY WITH SPECTROFLUORIMETRIC DETECTION FOR DETERMINATION OF HUMAN MILK RETINOL. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2001;59(6):86-91. Available from: https://sid.ir/paper/531723/en

IEEE: Copy

H.R. FALAHAT PISHEH, and A. GHARAVI NOORI, “APPLICATION OF HIGHT PERFORMANCE LIQUID CHROMATOGRAPHY WITH SPECTROFLUORIMETRIC DETECTION FOR DETERMINATION OF HUMAN MILK RETINOL,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 59, no. 6, pp. 86–91, 2001, [Online]. Available: https://sid.ir/paper/531723/en

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