EXTRACTION AND PURIFICATION OF PYOCIN 82 FROM AN ENDEMIC PSEUDOMONAS AERUGINOSA, ISOLATE 42 A

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ABDI AALI A.; MALEKZADEH F.; DIZAJI A.A.KH.; BEHZADIANNEZHAD GH.; HOSSEINI JAZANI N.

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Journal Paper

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Journal: KOWSAR MEDICAL JOURNAL Year:2002 | Volume:7 | Issue:part 3 Page(s): 207-214

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Title

EXTRACTION AND PURIFICATION OF PYOCIN 82 FROM AN ENDEMIC PSEUDOMONAS AERUGINOSA, ISOLATE 42 A

Author(s)

Keywords

PSEUDOMONAS AERUGINOSA

PYOCIN S2

PURIFICATION

Abstract

A collection of 82 wild type P. aeruginosa were obtained from different clinical sources (such as blood, stool, ear, burn and etc. . .). Pyocin typing of P. aeruginosa strains was performed as described by Fyfe and colleagues. PYOCIN S2, a protease sensitive bacteriocin was produced by one of the endemic isolate which was named P.aeruginosa 42 A. PYOCIN S2 production was induced by mitomycin C in an isolate which was able to produce the PYOCIN S2 in a small amount. The bacterial lysate was treated with 80% amonium sulfate solution and then proteins were precipitated by centrifugation. Pyocin S2 was purified by using ion exchange chromatography with CM-Sepharose CL-6B. Pyocin activity of the fractions was detected using the Govan spot testing method. The purity of active fraction was confirmed by using SDS- PAGE (10% acrylamide gel, staining with silver nitrate and coomassie stains). The results showed that one strain among the isolates produced PYOCIN S2. Mitomycin C (2 µg/m) induced the PYOCIN S2 production and increased its activity in this strain. In electrophoretic pattern (SDS - PAGE) a 74 KD single band was detected, indicating the purity of the PYOCIN S2 obtained and the precision of the extraction method.

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APA: Copy

ABDI AALI, A., MALEKZADEH, F., DIZAJI, A.A.KH., BEHZADIANNEZHAD, GH., & HOSSEINI JAZANI, N.. (2002). EXTRACTION AND PURIFICATION OF PYOCIN 82 FROM AN ENDEMIC PSEUDOMONAS AERUGINOSA, ISOLATE 42 A. KOWSAR MEDICAL JOURNAL, 7(part 3), 207-214. SID. https://sid.ir/paper/532120/en

Vancouver: Copy

ABDI AALI A., MALEKZADEH F., DIZAJI A.A.KH., BEHZADIANNEZHAD GH., HOSSEINI JAZANI N.. EXTRACTION AND PURIFICATION OF PYOCIN 82 FROM AN ENDEMIC PSEUDOMONAS AERUGINOSA, ISOLATE 42 A. KOWSAR MEDICAL JOURNAL[Internet]. 2002;7(part 3):207-214. Available from: https://sid.ir/paper/532120/en

IEEE: Copy

A. ABDI AALI, F. MALEKZADEH, A.A.KH. DIZAJI, GH. BEHZADIANNEZHAD, and N. HOSSEINI JAZANI, “EXTRACTION AND PURIFICATION OF PYOCIN 82 FROM AN ENDEMIC PSEUDOMONAS AERUGINOSA, ISOLATE 42 A,” KOWSAR MEDICAL JOURNAL, vol. 7, no. part 3, pp. 207–214, 2002, [Online]. Available: https://sid.ir/paper/532120/en

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