PCR AMPLIFICATION, CLONING AND MOLECULAR CHARACTERIZATION OF A GRMA HOMOLOGOUS GENE WHICH HAS HIGH HOMOLOGY TO SODIUM-PROTON ANTIPORTER (NAPA) GENE OF ENTEROCOCCUS HIREA

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BEHRAVAN J.; MOIR A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

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Journal: Iranian Journal of Basic Medical Sciences Year:2000 | Volume:3 | Issue:1 (6) Page(s): 4-10

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Title

PCR AMPLIFICATION, CLONING AND MOLECULAR CHARACTERIZATION OF A GRMA HOMOLOGOUS GENE WHICH HAS HIGH HOMOLOGY TO SODIUM-PROTON ANTIPORTER (NAPA) GENE OF ENTEROCOCCUS HIREA

Author(s)

BEHRAVAN J. | MOIR A. | Issue Writer Certificate

Keywords

GRMA

BACILLUS MEGATERIUM

BACILLUS CEREUS

NAPA

Abstract

The gene GRMA of BACILLUS MEGATERIUM ATCC 12872 encodes a protein responsible for early spore germination events. In order to determine whether a homologuous of the GRMA is present in B. cereus chromosome, a PCR cloning strategy with degenerate primers was exploited. The amplicon with the expected size was purified, cloned into two different B. subtilis integrational vectors, and sequenced. Analysis of the cloned DNA using tBLASTn revealed high homology to B. magaterium GRMA. This is the first report of identification of a GRMA homologue in B. cereus. We suggest that GRMA genes family has a common function in spore germination of both B. megaterium and B. cereus. It would be interesting to investigate the role of an antiporter in the complicated process of spore germination.

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APA: Copy

BEHRAVAN, J., & MOIR, A.. (2000). PCR AMPLIFICATION, CLONING AND MOLECULAR CHARACTERIZATION OF A GRMA HOMOLOGOUS GENE WHICH HAS HIGH HOMOLOGY TO SODIUM-PROTON ANTIPORTER (NAPA) GENE OF ENTEROCOCCUS HIREA. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 3(1 (6)), 4-10. SID. https://sid.ir/paper/533863/en

Vancouver: Copy

BEHRAVAN J., MOIR A.. PCR AMPLIFICATION, CLONING AND MOLECULAR CHARACTERIZATION OF A GRMA HOMOLOGOUS GENE WHICH HAS HIGH HOMOLOGY TO SODIUM-PROTON ANTIPORTER (NAPA) GENE OF ENTEROCOCCUS HIREA. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2000;3(1 (6)):4-10. Available from: https://sid.ir/paper/533863/en

IEEE: Copy

J. BEHRAVAN, and A. MOIR, “PCR AMPLIFICATION, CLONING AND MOLECULAR CHARACTERIZATION OF A GRMA HOMOLOGOUS GENE WHICH HAS HIGH HOMOLOGY TO SODIUM-PROTON ANTIPORTER (NAPA) GENE OF ENTEROCOCCUS HIREA,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 3, no. 1 (6), pp. 4–10, 2000, [Online]. Available: https://sid.ir/paper/533863/en

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