EFFECTS OF GLIBENCLAMIDE ON INSULIN SECRETION AND GLUCOKINASE ACTIVITY IN NORMAL AND DIABETIC RAT PANCREATIC ISLETS

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KHAYATIAN M.; ARDESHIR LARIJANI MOHAMMAD BAGHER; FARZAMI B.; BOUSHEHRI H.; POURNOURMOHAMMADI SH.

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Journal: Iranian Journal of Diabetes and Metabolism Year:2006 | Volume:6 | Issue:1 Page(s): 17-26

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Title

EFFECTS OF GLIBENCLAMIDE ON INSULIN SECRETION AND GLUCOKINASE ACTIVITY IN NORMAL AND DIABETIC RAT PANCREATIC ISLETS

Author(s)

Keywords

GLIBENCLAMIDEQ3

PANCREATIC ISLETSQ3

ISLET ISOLATIONQ3

INSULIN SECRETIONQ3

GLUCOKINASEQ3

NSTZ-DIABETIC RATSQ3

Abstract

Background: Sulfonylurea agents such as GLIBENCLAMIDE (Glyburide) have been widely prescribe in treatment of type 2 diabetic patients for decades, but controversy remains about their precise mechanism of action. On the other hand, GLUCOKINASE serves as a glucose sensor in pancreatic b-cells and plays a key role in the regulation of INSULIN SECRETION and glucose homeostasis. The aim of the present study was to evaluate the effect of GLIBENCLAMIDE on INSULIN SECRETION and GLUCOKINASE activity in the rat isolated PANCREATIC ISLETS of Langerhans.Methods: The islets from normal and type 2 diabetic rats were isolated by collagenase digestion method. GLUCOKINASE activity was measured via determination the rate of glucose-6-phosphate formation in the fluorometric assay. INSULIN SECRETION from hand-picked islets was evaluated by static incubation technique. Insulin concentration was measured by rat insulin ELISA kit.Results: Our findings obtained from incubation of Glybenclamide with PANCREATIC ISLETS revealed that this agent increases basal INSULIN SECRETION (at 2.8 mM glucose) in both normal and diabetic rats as compared it with control islet (without drug). However, the increase of INSULIN SECRETION in response to 16.7 mM glucose was not significant. On the other hand, Glybenclamide had no activating and/or inhibiting effect on pancreatic GLUCOKINASE activity in both diabetic and normal Rats. But reduced activity of this enzyme in diabetic rats was significant in comparison with normal.Conclusion: These data show that increasing effect of Glybenclamide on INSULIN SECRETION is through a mechanism other than affecting Glucose Mediated Insulin Release. Moreover, the regulation of pancreatic GLUCOKINASE does not depend on glybenclamid.

Cites

EFFECT OF OLIVE LEAF (OLEA EUROPAEA L.) ON GLUCOSE-STIMULATED INSULIN SECRETION FROM ISOLATED PANCREATIC ISLETS OF RAT

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APA: Copy

KHAYATIAN, M., ARDESHIR LARIJANI, MOHAMMAD BAGHER, FARZAMI, B., BOUSHEHRI, H., & POURNOURMOHAMMADI, SH.. (2006). EFFECTS OF GLIBENCLAMIDE ON INSULIN SECRETION AND GLUCOKINASE ACTIVITY IN NORMAL AND DIABETIC RAT PANCREATIC ISLETS. JOURNAL OF DIABETES AND METABOLIC DISORDERS, 6(1), 17-26. SID. https://sid.ir/paper/53976/en

Vancouver: Copy

KHAYATIAN M., ARDESHIR LARIJANI MOHAMMAD BAGHER, FARZAMI B., BOUSHEHRI H., POURNOURMOHAMMADI SH.. EFFECTS OF GLIBENCLAMIDE ON INSULIN SECRETION AND GLUCOKINASE ACTIVITY IN NORMAL AND DIABETIC RAT PANCREATIC ISLETS. JOURNAL OF DIABETES AND METABOLIC DISORDERS[Internet]. 2006;6(1):17-26. Available from: https://sid.ir/paper/53976/en

IEEE: Copy

M. KHAYATIAN, MOHAMMAD BAGHER ARDESHIR LARIJANI, B. FARZAMI, H. BOUSHEHRI, and SH. POURNOURMOHAMMADI, “EFFECTS OF GLIBENCLAMIDE ON INSULIN SECRETION AND GLUCOKINASE ACTIVITY IN NORMAL AND DIABETIC RAT PANCREATIC ISLETS,” JOURNAL OF DIABETES AND METABOLIC DISORDERS, vol. 6, no. 1, pp. 17–26, 2006, [Online]. Available: https://sid.ir/paper/53976/en

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