ULTRA STRUCTURAL STUDY OF MICE EMBRYOS OBTAINED FROM YOUNG AND OLD OOCYTES

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SHAHVERDI A.; MOVAHEDIAN M.; REZAZADEH VALOUJERDI M.; KAZEMI ASHTIANI S.

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Journal Paper

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Journal: Anatomical Sciences Journal Year:2006 | Volume:4 | Issue:1 Page(s): 19-28

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Title

ULTRA STRUCTURAL STUDY OF MICE EMBRYOS OBTAINED FROM YOUNG AND OLD OOCYTES

Author(s)

SHAHVERDI A. | MOVAHEDIAN M. | REZAZADEH VALOUJERDI M. | KAZEMI ASHTIANI S. | Issue Writer Certificate

Keywords

ULTRASTRUCTURE

OLD AND YOUNG OOCYTE

CULTURE

FERTILIZATION

Abstract

Introduction: The aim of this study was to compare ultra structure of embryos obtained from young and old mouse oocytes.Materials and Methods: Metaphase II oocytes were retrieved from old NMRI mouse (7-9 months old) and young NMRI (10-12 weeks old) after ovarian stimulation. NMRI mouse sperms (10-12 weeks old) were added to T6 medium containing retrieved oocytes.After PN formation, embryos were transferred to CULTURE medium (comtaining 15mg/ml BSA).Developmenting of embryos was recorded every 24 hours. 2 cell embryos after were fined in 2.5% glutardehid phosphate the samples dehydrated and embedded. Blokcs were sliced after timming. Seim thin (500 nm) and ultra thin (70 nm) sections had been (collected on copper grids) stained. Samples were studied by election microscopy.Results: At the (LM) level, embryos driven from young and old oocytes were surrounded by zona pellocida polar bodies and cellular fragmentation were seen in perivitelline space which was morefrequent in older embryos.In embryos driven from old oocytes, perivittelline space was more enpanded and microvillies were arragned reqularly. Nuclei of blastocytes were enchromatin in both embroyos. In old embryos, intramuscular anulatellae lamllae was ween which was secndary to folding of nucleous inhere membra.Mithochondies and intermediate filaments were seen in both embryos in cytoplasm. Which were similar in morphology and ultra structure. But their arrangement and distribution were different.In embryos dreven from oocytes. Most of mitochonderies were central and around the nucleuses.While in young embryos, they were distributed in cytoplasm.SER collection was higher in young embryos while residual bodies and lipid were more frequent in old embryos.Conclusion: This study revels that embryos driven from old oocytes had several ultra structure changes in comparison to young embryos. This may be one of the reason follow implantation and birth rate in old subjects.

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APA: Copy

SHAHVERDI, A., MOVAHEDIAN, M., REZAZADEH VALOUJERDI, M., & KAZEMI ASHTIANI, S.. (2006). ULTRA STRUCTURAL STUDY OF MICE EMBRYOS OBTAINED FROM YOUNG AND OLD OOCYTES. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 4(1), 19-28. SID. https://sid.ir/paper/551132/en

Vancouver: Copy

SHAHVERDI A., MOVAHEDIAN M., REZAZADEH VALOUJERDI M., KAZEMI ASHTIANI S.. ULTRA STRUCTURAL STUDY OF MICE EMBRYOS OBTAINED FROM YOUNG AND OLD OOCYTES. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2006;4(1):19-28. Available from: https://sid.ir/paper/551132/en

IEEE: Copy

A. SHAHVERDI, M. MOVAHEDIAN, M. REZAZADEH VALOUJERDI, and S. KAZEMI ASHTIANI, “ULTRA STRUCTURAL STUDY OF MICE EMBRYOS OBTAINED FROM YOUNG AND OLD OOCYTES,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 4, no. 1, pp. 19–28, 2006, [Online]. Available: https://sid.ir/paper/551132/en

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