POSTER PRESENTATIONS; PRODUCTION AND TRANSDUCTION OF A RECOMBINANT LENTIVIRAL VECTOR CARRYING EGFP GENE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ALIZADEH SANI H.; CHEGINI R.; KHORAM KHOURSHID H.; AKHOUNDI M.A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2010 | Volume:12 | Issue:SUPPLEMENT 1 (6TH CONGRESS ON STEM CELL BIOLOGY AND TECHNOLOGY) Page(s): 24-24

Download Full-Text

View:

285

Download:

Cites:

Information Journal Paper

Title

POSTER PRESENTATIONS; PRODUCTION AND TRANSDUCTION OF A RECOMBINANT LENTIVIRAL VECTOR CARRYING EGFP GENE

Author(s)

ALIZADEH SANI H. | CHEGINI R. | KHORAM KHOURSHID H. | AKHOUNDI M.A.

Keywords

LENTIVIRUS

EGFP REPORTER GENE

HEK293FT CELL LINE

TRANSFECTION

TRANSDUCTION

Abstract

Objective: Lentivector mediated production of EGFP carrying murine spermatogonial stem cells.Materials and Methods: EGFP gene was subloned into plenti6v5/dest vector. The transgene and/or the packaging vectors were cloned and purified using JM109 bacterial cells. Calcium phosphate TRANSFECTION using HEK293FT CELL LINE was performed to produce the EGFP carrying lentivectors. Spermatogonial stem cells were then transduced with the EGFP carrying lentivectors.Results: PCR and Sequencing confirmed the integration of EGFP in to the lentiviral vector. TRANSDUCTION with the 24 hour supernatant demonstrated a better result than 48 hour supernatant. Green color in spermatogonial cells under the UV light after cell passage illustrated the maintenance and expression of EGFP which indicates the entrance of EGFP gene into the cell genome.Conclusion: By means of recombinant lentiviral vectors it is possible to integrate the gene of interest into the cell genome. This can lead to the final production of transgenic animals

Multimedia

No record.

Cites

No record.

References

No record.

Cite

APA: Copy

ALIZADEH SANI, H., CHEGINI, R., KHORAM KHOURSHID, H., & AKHOUNDI, M.A.. (2010). POSTER PRESENTATIONS; PRODUCTION AND TRANSDUCTION OF A RECOMBINANT LENTIVIRAL VECTOR CARRYING EGFP GENE. CELL JOURNAL (YAKHTEH), 12(SUPPLEMENT 1 (6TH CONGRESS ON STEM CELL BIOLOGY AND TECHNOLOGY)), 24-24. SID. https://sid.ir/paper/557316/en

Vancouver: Copy

ALIZADEH SANI H., CHEGINI R., KHORAM KHOURSHID H., AKHOUNDI M.A.. POSTER PRESENTATIONS; PRODUCTION AND TRANSDUCTION OF A RECOMBINANT LENTIVIRAL VECTOR CARRYING EGFP GENE. CELL JOURNAL (YAKHTEH)[Internet]. 2010;12(SUPPLEMENT 1 (6TH CONGRESS ON STEM CELL BIOLOGY AND TECHNOLOGY)):24-24. Available from: https://sid.ir/paper/557316/en

IEEE: Copy

H. ALIZADEH SANI, R. CHEGINI, H. KHORAM KHOURSHID, and M.A. AKHOUNDI, “POSTER PRESENTATIONS; PRODUCTION AND TRANSDUCTION OF A RECOMBINANT LENTIVIRAL VECTOR CARRYING EGFP GENE,” CELL JOURNAL (YAKHTEH), vol. 12, no. SUPPLEMENT 1 (6TH CONGRESS ON STEM CELL BIOLOGY AND TECHNOLOGY), pp. 24–24, 2010, [Online]. Available: https://sid.ir/paper/557316/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops