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Title

POSTER PRESENTATIONS; IN VITRO DIFFERENTIATION OF BLASTEMA CELLS DERIVED FROM NEW ZEALAND WHITE RABBIT PINNA INTO INSULIN PRODUCING CELLS

Pages

  43-43

Abstract

 After injury, in some animals, blastema tissue is formed which helps in restoration and healing of the injured tissue. One probability for production of these cells is dedifferentiation of mesenchymal cells to undifferentiated cells that can divide and differentiate to various cell types. In this study, New Zealand white rabbit pinnas were punched and after the second punch, a thin ring containing BLASTEMA CELLS was transferred into culture medium. Cells derived from this tissue were grown and then induced to differentiate into INSULIN PRODUCING CELLS by culturing in serum free medium containing high glucose concentration, nicotinamide and b-mercaptoethanol.Dithizone staining and measuring the concentration of insulin in culture medium showed that these cells were able to produce insulin from two days after induction. Also the defferentiated cells produced some clusters of cells similar to islets of langerhans within one week to one month of treatment. Finally, RT-PCR analyseis showed that expression of insulin mRNA was induced in these cells.These results demonstrate high capacity of BLASTEMA CELLS to differentiate into INSULIN PRODUCING CELLS and therefore they might be useful in finding novel approaches or new cell source with the potential for CELL THERAPY.

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    APA: Copy

    SAEINASAB, M., MOGHADAM MATIN, M., & BAHRAMI, A.R.. (2010). POSTER PRESENTATIONS; IN VITRO DIFFERENTIATION OF BLASTEMA CELLS DERIVED FROM NEW ZEALAND WHITE RABBIT PINNA INTO INSULIN PRODUCING CELLS. CELL JOURNAL (YAKHTEH), 12(SUPPLEMENT 1 (6TH CONGRESS ON STEM CELL BIOLOGY AND TECHNOLOGY)), 43-43. SID. https://sid.ir/paper/557327/en

    Vancouver: Copy

    SAEINASAB M., MOGHADAM MATIN M., BAHRAMI A.R.. POSTER PRESENTATIONS; IN VITRO DIFFERENTIATION OF BLASTEMA CELLS DERIVED FROM NEW ZEALAND WHITE RABBIT PINNA INTO INSULIN PRODUCING CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2010;12(SUPPLEMENT 1 (6TH CONGRESS ON STEM CELL BIOLOGY AND TECHNOLOGY)):43-43. Available from: https://sid.ir/paper/557327/en

    IEEE: Copy

    M. SAEINASAB, M. MOGHADAM MATIN, and A.R. BAHRAMI, “POSTER PRESENTATIONS; IN VITRO DIFFERENTIATION OF BLASTEMA CELLS DERIVED FROM NEW ZEALAND WHITE RABBIT PINNA INTO INSULIN PRODUCING CELLS,” CELL JOURNAL (YAKHTEH), vol. 12, no. SUPPLEMENT 1 (6TH CONGRESS ON STEM CELL BIOLOGY AND TECHNOLOGY), pp. 43–43, 2010, [Online]. Available: https://sid.ir/paper/557327/en

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