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Information Journal Paper

Title

THE EFFECTS OF EXENDINE-4 ON INSULIN PRODUCING CELL DIFFERENTIATION FROM RAT BONE MARROW-DERIVED MESENCHYMAL STEM CELLS

Pages

  187-194

Abstract

 Objective: The aim of this study was to evaluate the effect of EXENDIN-4 (EX-4) on differentiation of INSULIN-PRODUCING CELLS (IPCs) from rat bone marrow-derived MESENCHYMAL STEM CELLS (RAT-BM-MSCs).Materials and Methods: In this experimental study, RAT-BM-MSCs were cultured and the cells characterized by flow cytometry analysis of cell surface markers. RAT-BM-MSCs were subsequently treated with induction media with or without EX-4. After induction, the presence of IPCs was demonstrated with dithizone (DTZ) staining and gene expression profiles for pancreatic cell differentiation markers (PDX-1, GLUT-2, insulin) were assessed using reverse transcription polymerase chain reaction (RT-PCR). Insulin excreted from differentiated cells was analyzed with radioimmunoassay (RIA). The two-tailed student's t-test was used for comparison of the obtained values.Results: The percentage of DTZ-positive cells significantly increased in EX-4 treated cells (p<0.05). Expression of the islet-associated genes PDX-1, GLUT-2 and insulin genes in EX-4 treated cells was markedly higher than in the cells exposed to differentiation media without EX-4. RIA analysis demonstrated significant release of insulin with the glucose challenge test in EX-4 treated cells compared to EX-4 untreated cells.Conclusion: The results of this study have demonstrated that EX-4 can enhance differentiation of IPCs from RAT-BM-MSCs.

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    APA: Copy

    NEJAD DEHBASHI, FERESHTEH, HASHEMITABAR, MAHMOUD, ORAZIZADEH, MAHMOUD, BAHRAMZADEH, SOMAIEH, SHAHHOSSEINI POURSHOUSHTARY, ELHAM, & KHORSANDI, LAYASADAT. (2014). THE EFFECTS OF EXENDINE-4 ON INSULIN PRODUCING CELL DIFFERENTIATION FROM RAT BONE MARROW-DERIVED MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH), 16(2 (62)), 187-194. SID. https://sid.ir/paper/652600/en

    Vancouver: Copy

    NEJAD DEHBASHI FERESHTEH, HASHEMITABAR MAHMOUD, ORAZIZADEH MAHMOUD, BAHRAMZADEH SOMAIEH, SHAHHOSSEINI POURSHOUSHTARY ELHAM, KHORSANDI LAYASADAT. THE EFFECTS OF EXENDINE-4 ON INSULIN PRODUCING CELL DIFFERENTIATION FROM RAT BONE MARROW-DERIVED MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2014;16(2 (62)):187-194. Available from: https://sid.ir/paper/652600/en

    IEEE: Copy

    FERESHTEH NEJAD DEHBASHI, MAHMOUD HASHEMITABAR, MAHMOUD ORAZIZADEH, SOMAIEH BAHRAMZADEH, ELHAM SHAHHOSSEINI POURSHOUSHTARY, and LAYASADAT KHORSANDI, “THE EFFECTS OF EXENDINE-4 ON INSULIN PRODUCING CELL DIFFERENTIATION FROM RAT BONE MARROW-DERIVED MESENCHYMAL STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 16, no. 2 (62), pp. 187–194, 2014, [Online]. Available: https://sid.ir/paper/652600/en

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