CALCITONIN GENE-RELATED PEPTIDE EFFECTS ON PHENOTYPE AND IL-12 PRODUCTION OF MONOCYTE-DERIVED DENDRITIC CELLS IN RHEUMATOID ARTHRITIS PATIENTS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MORAD ABBASI J.; RASTIN M.; REZAEI YAZDI Z.; MIRFEYZI Z.; MOAZENI S.M.; TABASI N.; BROUK A.; MAHMOUDI MAHMOUD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Basic Medical Sciences Year:2010 | Volume:13 | Issue:4 (47) Page(s): 161-169

Download Full-Text

View:

572

Download:

338

Cites:

Information Journal Paper

Title

CALCITONIN GENE-RELATED PEPTIDE EFFECTS ON PHENOTYPE AND IL-12 PRODUCTION OF MONOCYTE-DERIVED DENDRITIC CELLS IN RHEUMATOID ARTHRITIS PATIENTS

Author(s)

Keywords

CALCITONIN GENE-RELATED PEPTIDE

DENDRITIC CELL

IMMUNE TOLERANCE

RHEUMATOID ARTHRITIS

Abstract

Recent studies on human indicate that the introduction of therapeutic use of tolerogenic DENDRITIC CELL (DC) for chronic inflammatory conditions is imminent. For the purpose of defining CGRP potency in tolerogenic DC production, we investigated the phenotype and IL-12 production of DCs generated from the monocytes of RHEUMATOID ARTHRITIS (RA) patients in the presence of the CALCITONIN GENE-RELATED PEPTIDE (CGRP), as a multifunctional neuropeptide.Materials and Methods: DCs were generated from isolated monocytes from four resistant and two early female RA patients using IL- 4, GM-CSF, and CGRP at concentrations of 0, 1, and 100 nM. Then, the phenotype of neuropeptide-treated or untreated DCs was determined using flow cytometry and the IL-12 production was measured by ELISA.Results: Our study showed that, on the last day of the culture, at a concentration of 1 nM CGRP, the mean fluorescence intensity (MFI) for CD80 increased (14.13%) and the MFIs for CD83, CD86, and HLA-DR decreased (14.57%, 5.28%, and 6.88% respectively). Moreover, at 100 nM CGRP concentration, the MFI for CD80 increased (11.10%) and the MFIs for CD83, CD86, and HLA-DR decreased (4.27%, 18.60%, and 19.75% respectively). In addition, our results indicated that the mean concentrations of IL-12 produced at 0, 1, and 100 nm CGRP concentrations measured 13.72±2.41, 11.01±1.61, and 7±1.34 pg/ml respectively.Conclusion: Decreased CD83, CD86, and HLA-DR expression and reduced IL-12 production by CGRP were found in the RA patients' monocyte-derived DCs. CD83 is a well-defined DC activation marker. HLA-DR and CD86 are appropriate molecules for inducing an immune response. IL-12 promotes cell-mediated immunity. Therefore we suggest that CGRP may be used as an inducer in the production of tolerogenic DCs.

Cites

No record.

References

Cite

APA: Copy

MORAD ABBASI, J., RASTIN, M., REZAEI YAZDI, Z., MIRFEYZI, Z., MOAZENI, S.M., TABASI, N., BROUK, A., & MAHMOUDI, MAHMOUD. (2010). CALCITONIN GENE-RELATED PEPTIDE EFFECTS ON PHENOTYPE AND IL-12 PRODUCTION OF MONOCYTE-DERIVED DENDRITIC CELLS IN RHEUMATOID ARTHRITIS PATIENTS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 13(4 (47)), 161-169. SID. https://sid.ir/paper/557774/en

Vancouver: Copy

MORAD ABBASI J., RASTIN M., REZAEI YAZDI Z., MIRFEYZI Z., MOAZENI S.M., TABASI N., BROUK A., MAHMOUDI MAHMOUD. CALCITONIN GENE-RELATED PEPTIDE EFFECTS ON PHENOTYPE AND IL-12 PRODUCTION OF MONOCYTE-DERIVED DENDRITIC CELLS IN RHEUMATOID ARTHRITIS PATIENTS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2010;13(4 (47)):161-169. Available from: https://sid.ir/paper/557774/en

IEEE: Copy

J. MORAD ABBASI, M. RASTIN, Z. REZAEI YAZDI, Z. MIRFEYZI, S.M. MOAZENI, N. TABASI, A. BROUK, and MAHMOUD MAHMOUDI, “CALCITONIN GENE-RELATED PEPTIDE EFFECTS ON PHENOTYPE AND IL-12 PRODUCTION OF MONOCYTE-DERIVED DENDRITIC CELLS IN RHEUMATOID ARTHRITIS PATIENTS,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 13, no. 4 (47), pp. 161–169, 2010, [Online]. Available: https://sid.ir/paper/557774/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops