مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

GENETICALLY ENGINEERED MESENCHYMAL STEM CELLS STABLY EXPRESSING GREEN FLUORESCENT PROTEIN

Pages

  24-30

Abstract

 Objective(s): MESENCHYMAL STEM CELLS (MSCs) are nonhematopoietic stromal cells that are capable of differentiating into and contribute to the regeneration of mesenchymal tissues. Human MESENCHYMAL STEM CELLS (hMSCs) are ideal targets in cell transplantation and tissue engineering. Enhanced GREEN FLUORESCENT PROTEIN (EGFP) has been an important reporter gene for gene therapy. The aim of this study was establishment of MSCs expressing GFP.Materials and Methods: MSCs were isolated and characterized by Immunophenotyping. The pEGFP-N1 plasmid was extracted from previously transformed Escherichia. coli cells and transfected into MSCs using FuGENE HD TRANSFECTION reagent. Stable cells were established in the presence of geneticin. Expression of GFP was detected by RTPCR, western blot analysis and immunoflorecent microscope.Results: MSCs were successfully isolated and characterized. The MSCs transfected with the pEGFP-N1 plasmid expressed GFP both in mRNA and protein levels while cells transfected with empty vector did not. Conclusion: The results suggested that this engineered cell line will be used in the future studies and can easily be traced in vivo.

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    Cite

    APA: Copy

    HALABIAN, R., MOHAMMADI, MOHAMMAD HOSSEIN, SALIMI, MOHAMMAD, AMANI, MARYAM, MOHAMMADI ROUSHANDEH, A., AGHAEIPOUR, M., AMIRIZADEH, N., EBRAHIMI, MAJID, JAHANIAN NAJAFABADI, ALI, & HABIBI ROUDKENAR, M.. (2010). GENETICALLY ENGINEERED MESENCHYMAL STEM CELLS STABLY EXPRESSING GREEN FLUORESCENT PROTEIN. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 13(2 (45)), 24-30. SID. https://sid.ir/paper/558461/en

    Vancouver: Copy

    HALABIAN R., MOHAMMADI MOHAMMAD HOSSEIN, SALIMI MOHAMMAD, AMANI MARYAM, MOHAMMADI ROUSHANDEH A., AGHAEIPOUR M., AMIRIZADEH N., EBRAHIMI MAJID, JAHANIAN NAJAFABADI ALI, HABIBI ROUDKENAR M.. GENETICALLY ENGINEERED MESENCHYMAL STEM CELLS STABLY EXPRESSING GREEN FLUORESCENT PROTEIN. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2010;13(2 (45)):24-30. Available from: https://sid.ir/paper/558461/en

    IEEE: Copy

    R. HALABIAN, MOHAMMAD HOSSEIN MOHAMMADI, MOHAMMAD SALIMI, MARYAM AMANI, A. MOHAMMADI ROUSHANDEH, M. AGHAEIPOUR, N. AMIRIZADEH, MAJID EBRAHIMI, ALI JAHANIAN NAJAFABADI, and M. HABIBI ROUDKENAR, “GENETICALLY ENGINEERED MESENCHYMAL STEM CELLS STABLY EXPRESSING GREEN FLUORESCENT PROTEIN,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 13, no. 2 (45), pp. 24–30, 2010, [Online]. Available: https://sid.ir/paper/558461/en

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