CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTORS ENCODING PEP CDNA WITH FLAG AND GST TAGS

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NAZARI JAHANTIGH M.; GHAEDI K.; TANHAEI S.; RABIEI FARZANEH; NASRESFAHANI MOHAMMAD HOSSEIN; BAHARVAND HOSSEIN; KARBALAEI KH.; MIROULIAEI M.

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Journal: RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE" Year:2008 | Volume:35 | Issue:6 Page(s): 75-88

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Title

CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTORS ENCODING PEP CDNA WITH FLAG AND GST TAGS

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Keywords

PEROXISOMEQ3

PEP (MOUSE PEROXISOMAL PROTEIN GENE)Q3

PROKARYOTIC EXPRESSION VECTORQ3

EUKARYOTIC EXPRESSION VECTORQ3

Abstract

PEROXISOMEs are single membrane eukaryotic organelles that perform variable functions depending on the tissue, the developmental stage or environmental conditions. Peroxisomal matrix proteins are synthesized in the cytoplasm and targeted to PEROXISOMEs by virtue of a peroxisomal targeting signal (PTS). One of the peroxisomal matrix proteins-Peroxisomal Protein (PEP)-has shown different pattern of expression in mouse embryo in various tissues, but the reason is unclear. PEP-cDNA was sub-cloned in pGEX-6p-2 PROKARYOTIC EXPRESSION VECTOR in order to label this gene with GST to purify PEP protein for further biochemical analysis and identifying related proteins later. PEP-cDNA was inserted downstream of FLAG gene and then inserted in pUcD2SRMCSHyg EUKARYOTIC EXPRESSION VECTOR to express tagged-PEP protein for transient transfection analysis and identifying localization of PEP protein. Our results clearly demonstrated that PEP and FLAG-PEP were inserted into the vectors appropriately and they were free from mutation.

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APA: Copy

NAZARI JAHANTIGH, M., GHAEDI, K., TANHAEI, S., RABIEI, FARZANEH, NASRESFAHANI, MOHAMMAD HOSSEIN, BAHARVAND, HOSSEIN, KARBALAEI, KH., & MIROULIAEI, M.. (2008). CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTORS ENCODING PEP CDNA WITH FLAG AND GST TAGS. RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE", 35(6), 75-88. SID. https://sid.ir/paper/55929/en

Vancouver: Copy

NAZARI JAHANTIGH M., GHAEDI K., TANHAEI S., RABIEI FARZANEH, NASRESFAHANI MOHAMMAD HOSSEIN, BAHARVAND HOSSEIN, KARBALAEI KH., MIROULIAEI M.. CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTORS ENCODING PEP CDNA WITH FLAG AND GST TAGS. RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE"[Internet]. 2008;35(6):75-88. Available from: https://sid.ir/paper/55929/en

IEEE: Copy

M. NAZARI JAHANTIGH, K. GHAEDI, S. TANHAEI, FARZANEH RABIEI, MOHAMMAD HOSSEIN NASRESFAHANI, HOSSEIN BAHARVAND, KH. KARBALAEI, and M. MIROULIAEI, “CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTORS ENCODING PEP CDNA WITH FLAG AND GST TAGS,” RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE", vol. 35, no. 6, pp. 75–88, 2008, [Online]. Available: https://sid.ir/paper/55929/en

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