STUDY OF HUMAN CHONDROCYTE REDIFFERNTIATION CAPACITY IN THREE-DIMENSIONAL HYDROGEL CULTURE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ESFANDIARI EBRAHIM; SHAKIBAEI M.; AMIRPOUR N.; FESHARAKI M.; NASRESFAHANI MOHAMMAD HOSSEIN; MOULAVI FARIBA; NAZEM KH.A.; DEHGHANI M.H.; CSAKI C.; RAZAVI SHAHNAZ; MOULAVI FARAHNAZ

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Basic Medical Sciences Year:2008 | Volume:11 | Issue:3 (39) Page(s): 152-158

Download Full-Text

View:

408

Download:

181

Cites:

Information Journal Paper

Title

STUDY OF HUMAN CHONDROCYTE REDIFFERNTIATION CAPACITY IN THREE-DIMENSIONAL HYDROGEL CULTURE

Author(s)

Keywords

ALGINATE

AUTOLOGOUS

CHONDROCYTES

COLLAGEN TYPE II

PROTEOGLYCAN

TRANSPLANTATION

Abstract

Objective(s): Articular cartilage tissue defects cannot be repaired by the proliferation of resident CHONDROCYTES. AUTOLOGOUS chondrocyte TRANSPLANTATION (ACT) is a relatively new therapeutic approach to cover full thickness articular cartilage defects by in vitro grown CHONDROCYTES from the joint of a patient. Therefore, we investigated the redifferentiation capability of human CHONDROCYTES maintained in ALGINATE culture. Materials and Methods: The cartilage specimens obtained from 50 patients who underwent total knee and hip operations at the teaching hospital of Isfahan University of Medical Sciences, Isfahan Iran. Isolated primary CHONDROCYTES were first grown in monolayer cultures for 1 to 6 passages (each passage lasting about 3 days). At each passage, monolayer cells seeded in ALGINATE culture and investigated morphologically and immunocytologically for expression of cartilage-specific markers (collagen type II and cartilage-specific PROTEOGLYCANs). Results: The CHONDROCYTES from monolayer passages P1 to P4 introduced in ALGINATE cultures regained a chondrocyte phenotype. Cells were interconnected by typical gap junctions and after few days, they produced a cartilagespecific extracellular matrix (collagen type II and cartilage-specific PROTEOGLYCANs). In contrast, cells from monolayer passages P5 and P6 did not redifferentiate to CHONDROCYTES in the ALGINATE cultures. Conclusion: Chondrocyte culture was established for the first time in Iran. The ALGINATE culture conditions promote the redifferentiation of dedifferentiated CHONDROCYTES that have still a chondrogenic potential. This procedure opens up a promising approach to produce sufficient numbers of differentiated CHONDROCYTES for ACT. Indeed, in some patients the harvested cells were used immediately and successfully for TRANSPLANTATION.

Cites

No record.

References

No record.

Cite

APA: Copy

ESFANDIARI, EBRAHIM, SHAKIBAEI, M., AMIRPOUR, N., FESHARAKI, M., NASRESFAHANI, MOHAMMAD HOSSEIN, MOULAVI, FARIBA, NAZEM, KH.A., DEHGHANI, M.H., CSAKI, C., RAZAVI, SHAHNAZ, & MOULAVI, FARAHNAZ. (2008). STUDY OF HUMAN CHONDROCYTE REDIFFERNTIATION CAPACITY IN THREE-DIMENSIONAL HYDROGEL CULTURE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 11(3 (39)), 152-158. SID. https://sid.ir/paper/560759/en

Vancouver: Copy

ESFANDIARI EBRAHIM, SHAKIBAEI M., AMIRPOUR N., FESHARAKI M., NASRESFAHANI MOHAMMAD HOSSEIN, MOULAVI FARIBA, NAZEM KH.A., DEHGHANI M.H., CSAKI C., RAZAVI SHAHNAZ, MOULAVI FARAHNAZ. STUDY OF HUMAN CHONDROCYTE REDIFFERNTIATION CAPACITY IN THREE-DIMENSIONAL HYDROGEL CULTURE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2008;11(3 (39)):152-158. Available from: https://sid.ir/paper/560759/en

IEEE: Copy

EBRAHIM ESFANDIARI, M. SHAKIBAEI, N. AMIRPOUR, M. FESHARAKI, MOHAMMAD HOSSEIN NASRESFAHANI, FARIBA MOULAVI, KH.A. NAZEM, M.H. DEHGHANI, C. CSAKI, SHAHNAZ RAZAVI, and FARAHNAZ MOULAVI, “STUDY OF HUMAN CHONDROCYTE REDIFFERNTIATION CAPACITY IN THREE-DIMENSIONAL HYDROGEL CULTURE,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 11, no. 3 (39), pp. 152–158, 2008, [Online]. Available: https://sid.ir/paper/560759/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops