IDENTIFICATION AND QUANTITATIVE DETERMINATION OF 4-HYDROXYISOLEUCINE IN TRIGONELLA FOENUM-GRAECUM L. FROM IRAN

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HAJI MAHDIPOUR H.; SADAT EBRAHIMI S.E.; AMANZADEH Y.; IZADDOUST M.; GIVI E.

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Journal: Journal of Medicinal Plants Year:2010 | Volume:9 | Issue:SUPPLEMENT 6 Page(s): 29-34

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Title

IDENTIFICATION AND QUANTITATIVE DETERMINATION OF 4-HYDROXYISOLEUCINE IN TRIGONELLA FOENUM-GRAECUM L. FROM IRAN

Author(s)

Keywords

TRIGONELLA FOENUM-GRAECUM

AMINO ACID

DIABETES

4-HYDROXYISOLEUCINE

Abstract

Background: The seeds of TRIGONELLA FOENUM-GRAECUM L. (Fenugreek) are used in Iranian traditional medicine as blood sugar lowering agent. According to last investigations, hypoglycemic property is related to the AMINO ACIDs of the plant specially 4-HYDROXYISOLEUCINE. Objective: In this research, identification and quantitative determination of 4-HYDROXYISOLEUCINE in Fenugreek seeds from Shahreza, central part of Iran, has been investigated.Methods: Powdered seeds of the plant were defatted and then extracted by using ethanol 50%. In order to isolation of 4-HYDROXYISOLEUCINE from the extract, ion exchange chromatography was used and the AMINO ACID content was determined by using high performance liquid chromatography technique. Results: The results showed the presence of 0.4% 4-HYDROXYISOLEUCINE in the plant seeds which is considerable amount of the AMINO ACID in the plant collected from Iran.Conclusion: According to the effects of 4-HYDROXYISOLEUCINE such as antihyperglycemic and antihyperlipidemic properties, fenugreek can be considered as an herbal remedy in supportive treatment of these disorders.

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APA: Copy

HAJI MAHDIPOUR, H., SADAT EBRAHIMI, S.E., AMANZADEH, Y., IZADDOUST, M., & GIVI, E.. (2010). IDENTIFICATION AND QUANTITATIVE DETERMINATION OF 4-HYDROXYISOLEUCINE IN TRIGONELLA FOENUM-GRAECUM L. FROM IRAN. JOURNAL OF MEDICINAL PLANTS, 9(SUPPLEMENT 6), 29-34. SID. https://sid.ir/paper/562099/en

Vancouver: Copy

HAJI MAHDIPOUR H., SADAT EBRAHIMI S.E., AMANZADEH Y., IZADDOUST M., GIVI E.. IDENTIFICATION AND QUANTITATIVE DETERMINATION OF 4-HYDROXYISOLEUCINE IN TRIGONELLA FOENUM-GRAECUM L. FROM IRAN. JOURNAL OF MEDICINAL PLANTS[Internet]. 2010;9(SUPPLEMENT 6):29-34. Available from: https://sid.ir/paper/562099/en

IEEE: Copy

H. HAJI MAHDIPOUR, S.E. SADAT EBRAHIMI, Y. AMANZADEH, M. IZADDOUST, and E. GIVI, “IDENTIFICATION AND QUANTITATIVE DETERMINATION OF 4-HYDROXYISOLEUCINE IN TRIGONELLA FOENUM-GRAECUM L. FROM IRAN,” JOURNAL OF MEDICINAL PLANTS, vol. 9, no. SUPPLEMENT 6, pp. 29–34, 2010, [Online]. Available: https://sid.ir/paper/562099/en

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