RAPID DETECTION OF ISONIAZID RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS BY A SINGLE MULTIPLEX ALLELE-SPECIFIC POLYMERASE CHAIN REACTION ASSAY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TAGHAVI KIMIA; FARINA PARISSA; VARAHRAM MOHAMMAD; SHEIKHOSLAMI FATEMEH MARYAM; AHMADI MOJTABA; KAZEMPOOR MEHDI; MASJEDI MOHAMMAD REZA; VELAYATI ALI AKBAR

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2011 | Volume:13 | Issue:2 (50) Page(s): 97-102

Download Full-Text

View:

431

Download:

236

Cites:

Information Journal Paper

Title

RAPID DETECTION OF ISONIAZID RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS BY A SINGLE MULTIPLEX ALLELE-SPECIFIC POLYMERASE CHAIN REACTION ASSAY

Author(s)

Keywords

MULTIDRUG RESISTANT

TUBERCULOSIS

PCR

Abstract

Objective: Global surveillance has shown that drug resistant (DR) TUBERCULOSIS (TB) is widespread. Prompt detection of Mycobacterium TUBERCULOSIS drug resistance is essential for effective control of TB. The most frequent mutations associated with Isoniazid (INH) resistance in Mycobacterium are substitutions at codons 315 in the katG gene and the mabA-inhA promoter region (-15). This survey evaluated INH resistant-associated mutations in order to determine rapid detection of TB resistance.Materials and Methods: Through a descriptive cross- sectional study total of 96 sputum specimens were digested, examined microscopically for acid-fast bacilli and inoculated into Löwenstein-Jensen slants. Thereafter, the susceptibility and identification tests were performed on culture positive specimens. Subsequently, the strains were subjected to multiplex allele-specific polymerase chain reaction (MAS-PCR) targeting in the codons 315 in the katG gene and the mabA-inhA promoter region. Distinct PCR banding patterns were observed for different mutation profiles.Results: Drug susceptibility testing revealed that out of 96 available isolates, 30 (31.3%) were susceptible, 36 (37.5%) had multi-drug resistance (MDR-TB) and 30 (31.3%) showed mono- drug resistance. In comparison with the culture-based phenotypic drug susceptibility test, the sensitivity and specificity of MAS-PCR assay for drug resistance-related genetic mutations were 76.7% and 71.4%, respectively. The correlation between MAS-PCR and culture-based phenotypic drug susceptibility testing findings was 99.4%.Conclusion: The profile of the isolates suggests a significant number of different DR strains with a high frequency of mutations at codon 315 of the katG gene. MAS-PCR provides a rapid, simple and cost-effective method for detecting MDR-TB.

Cites

No record.

References

No record.

Cite

APA: Copy

TAGHAVI, KIMIA, FARINA, PARISSA, VARAHRAM, MOHAMMAD, SHEIKHOSLAMI, FATEMEH MARYAM, AHMADI, MOJTABA, KAZEMPOOR, MEHDI, MASJEDI, MOHAMMAD REZA, & VELAYATI, ALI AKBAR. (2011). RAPID DETECTION OF ISONIAZID RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS BY A SINGLE MULTIPLEX ALLELE-SPECIFIC POLYMERASE CHAIN REACTION ASSAY. CELL JOURNAL (YAKHTEH), 13(2 (50)), 97-102. SID. https://sid.ir/paper/563888/en

Vancouver: Copy

TAGHAVI KIMIA, FARINA PARISSA, VARAHRAM MOHAMMAD, SHEIKHOSLAMI FATEMEH MARYAM, AHMADI MOJTABA, KAZEMPOOR MEHDI, MASJEDI MOHAMMAD REZA, VELAYATI ALI AKBAR. RAPID DETECTION OF ISONIAZID RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS BY A SINGLE MULTIPLEX ALLELE-SPECIFIC POLYMERASE CHAIN REACTION ASSAY. CELL JOURNAL (YAKHTEH)[Internet]. 2011;13(2 (50)):97-102. Available from: https://sid.ir/paper/563888/en

IEEE: Copy

KIMIA TAGHAVI, PARISSA FARINA, MOHAMMAD VARAHRAM, FATEMEH MARYAM SHEIKHOSLAMI, MOJTABA AHMADI, MEHDI KAZEMPOOR, MOHAMMAD REZA MASJEDI, and ALI AKBAR VELAYATI, “RAPID DETECTION OF ISONIAZID RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS BY A SINGLE MULTIPLEX ALLELE-SPECIFIC POLYMERASE CHAIN REACTION ASSAY,” CELL JOURNAL (YAKHTEH), vol. 13, no. 2 (50), pp. 97–102, 2011, [Online]. Available: https://sid.ir/paper/563888/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops