مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

ROR2 PROMOTER METHYLATION CHANGE IN OSTEOBLASTIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS

Pages

  11-18

Abstract

 Objective: Osteoblasts arise from multipotent MESENCHYMAL STEM CELLs (MSCs) present in the bone marrow stroma and undergo further differentiation to osteocytes or bone cells.Many factors such as proteins present in the Wnt signaling pathway affect OSTEOBLAST DIFFERENTIATION. ROR2 is an orphan tyrosine kinase receptor that acts as a co-receptor in the non-canonical Wnt signaling pathway. However, ROR2 has been shown to be regulated by both canonical and non-canonical Wnt signaling pathways. ROR2 expression increases during differentiation of MSCs to osteoblasts and then decreases as cells differentiate to osteocytes. On the other hand, research has shown that ROR2 changes MSC fate towards osteoblasts by inducing osteogenic transcription factor OSTERIX. Here we speculated whether ROR2 gene expression regulation during osteoblast ogenesis is epigenetically determined.Materials and Methods: MSCs from bone marrow were isolated, expanded and characterized in vitro according to standard procedures. ROR2 promoter METHYLATION status was determined using METHYLATION specific PCR in a multipotent state and during differentiation to osteoblasts.Results: We determined that the demethylation process in ROR2 promoter occurs during the differentiation process. The process of demethylation begins at day 8 and continues until 21 days of differentiation.Conclusion: This result is in concordance with previous works on the role of ROR2 on OSTEOBLAST DIFFERENTIATION, which have shown an upregulation of ROR2 expression during this process.

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    APA: Copy

    TARFIEI, GORBANALI, NORUZINIA, MEHRDAD, SOLEIMANI, MASOOD, KAVIANI, SAEED, MAHMOODINIA MAYMAND, MARYAM, FARSHDOUSTI HAGH, MAJID, & PUJOL, PASCAL. (2011). ROR2 PROMOTER METHYLATION CHANGE IN OSTEOBLASTIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH), 13(1 (49)), 11-18. SID. https://sid.ir/paper/565487/en

    Vancouver: Copy

    TARFIEI GORBANALI, NORUZINIA MEHRDAD, SOLEIMANI MASOOD, KAVIANI SAEED, MAHMOODINIA MAYMAND MARYAM, FARSHDOUSTI HAGH MAJID, PUJOL PASCAL. ROR2 PROMOTER METHYLATION CHANGE IN OSTEOBLASTIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2011;13(1 (49)):11-18. Available from: https://sid.ir/paper/565487/en

    IEEE: Copy

    GORBANALI TARFIEI, MEHRDAD NORUZINIA, MASOOD SOLEIMANI, SAEED KAVIANI, MARYAM MAHMOODINIA MAYMAND, MAJID FARSHDOUSTI HAGH, and PASCAL PUJOL, “ROR2 PROMOTER METHYLATION CHANGE IN OSTEOBLASTIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 13, no. 1 (49), pp. 11–18, 2011, [Online]. Available: https://sid.ir/paper/565487/en

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