مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

DIFFERENT METHYLATION PATTERNS OF RUNX2, OSX, DLX5 AND BSP IN OSTEOBLASTIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS

Pages

  71-82

Abstract

 Objective: Runt-related transcription factor 2 (RUNX2) and osterix (OSX) as two specific osteoblast transcription factors and distal-less homeobox 5 (DLX5) as a non-specific one are of paramount importance in regulating osteoblast related genes including osteocalcin, bone sialoprotein (BSP), osteopontin and collagen type Ia1. The present study sets out to investigate whether epigenetic regulation of these genes is important in osteoblastic differentiation of MESENCHYMAL STEM CELLS (MSCs).Materials and Methods: In this experimental study, MSCs were differentiated to OSTEOBLASTS under the influence of the osteogenic differentiation medium. DNA and RNA were extracted at days 0, 7, 14 and 21 from MSCs differentiating to OSTEOBLASTS. Promoter regions of RUNX2, OSX, DLX5 and BSP were analyzed by methylation-specific PCR (MSP). Gene expression was analyzed during osteoblastic differentiation by quantitative real-time polymerase chain reaction (PCR).Results: MSP analysis revealed that promoter methylation status did not change in RUNX2, DLX5 and BSP during MSC osteoblastic differentiation. In contrast, OSX promoter showed a dynamic change in methylation pattern. Moreover, RUNX2, OSX, DLX5 and BSP promoter regions showed three different methylation patterns during MSC differentiation. Gene expression analyses confirmed these results.Conclusion: The results show that in differentiation of MSCs to OSTEOBLASTS, epigenetic regulation of OSX may play a leading role.

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References

Cite

APA: Copy

FARSHDOUSTI HAGH, MAJID, NORUZINIA, MEHRDAD, MORTAZAVI, YOUSEF, SOLEIMANI, MASOOD, KAVIANI, SAEED, ABROUN, SAEED, DEHGHANIFARD, ALI, & MAHMOODINIA MAYMAND, MARYAM. (2015). DIFFERENT METHYLATION PATTERNS OF RUNX2, OSX, DLX5 AND BSP IN OSTEOBLASTIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH), 17(1 (65)), 71-82. SID. https://sid.ir/paper/634664/en

Vancouver: Copy

FARSHDOUSTI HAGH MAJID, NORUZINIA MEHRDAD, MORTAZAVI YOUSEF, SOLEIMANI MASOOD, KAVIANI SAEED, ABROUN SAEED, DEHGHANIFARD ALI, MAHMOODINIA MAYMAND MARYAM. DIFFERENT METHYLATION PATTERNS OF RUNX2, OSX, DLX5 AND BSP IN OSTEOBLASTIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2015;17(1 (65)):71-82. Available from: https://sid.ir/paper/634664/en

IEEE: Copy

MAJID FARSHDOUSTI HAGH, MEHRDAD NORUZINIA, YOUSEF MORTAZAVI, MASOOD SOLEIMANI, SAEED KAVIANI, SAEED ABROUN, ALI DEHGHANIFARD, and MARYAM MAHMOODINIA MAYMAND, “DIFFERENT METHYLATION PATTERNS OF RUNX2, OSX, DLX5 AND BSP IN OSTEOBLASTIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 17, no. 1 (65), pp. 71–82, 2015, [Online]. Available: https://sid.ir/paper/634664/en

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