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Information Journal Paper

Title

T-BET MEDIATED ANTI-NEOPLASTIC EFFECTS OF DENDRITIC CELL-CYTOKINE INDUCED KILLER CELLS IN VITRO

Pages

  43-51

Abstract

 Objective: To investigate the molecular mechanism underlying T-bet mediated anti-neoplastic effects of cytokine induced killer (CIK) cells.Methods: Lymphocytes isolated from peripheral blood of leukemic children were induced with g- interferon (IFN-g), CD3McAb and interluki-2 (IL-2), and co-cultured with dendritic cells (DCs) to generate DC-CIK cells.The morphology and immunophenotype of these cells were determined by a light microscopy and flow cytometry, respectively. IL-2 and IFN-g levels released by DC-CIK cells were quantified by ELISA. CYTOTOXICITY of DC-CIK cells against leukemia cell lines was measured by MTT assay. FCM was used to detect CD4+CD25+Treg cells, while RT-PCR and Western blot were used to determine mRNA and protein expressions of FOXP3 and GATA3 in DC-CIK cells treated with T-bet monoclonal antibody.Findings: Induced DC-CIK cells were regular, round and transparent with variable cell volume and cellular aggregation.The main effector cells in this population were CD3+CD8+ cells and CD3+CD56+ cells.We demonstrated a time dependent increase in IL-2 and IFN-g levels after induction. DC-CIK cells were cytotoxic to B95 cells, Jhhan cells and M07e cells, with the highest CYTOTOXICITY towards B95 cells. Treatment with mouse anti-human T-bet monoclonal antibody resulted in an increase in the proportion of CD4+CD25+Treg cells and elevation of FOXP3 and GATA3 mRNA and protein levels.Conclusion: DC-CIK cells induced with cytokines were strongly cytotoxic towards a number of cancer cell lines.Foxp3 and GATA3 were implicated in the T-bet mediated anti-neoplastic effects of DC-CIK cells via activation of the Th1 pathway and suppression of the Th2 and Treg pathways.

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    APA: Copy

    MIAO, LIU, RUN MING, JIN, & YI, JIANG. (2012). T-BET MEDIATED ANTI-NEOPLASTIC EFFECTS OF DENDRITIC CELL-CYTOKINE INDUCED KILLER CELLS IN VITRO. IRANIAN JOURNAL OF PEDIATRICS, 22(1), 43-51. SID. https://sid.ir/paper/566691/en

    Vancouver: Copy

    MIAO LIU, RUN MING JIN, YI JIANG. T-BET MEDIATED ANTI-NEOPLASTIC EFFECTS OF DENDRITIC CELL-CYTOKINE INDUCED KILLER CELLS IN VITRO. IRANIAN JOURNAL OF PEDIATRICS[Internet]. 2012;22(1):43-51. Available from: https://sid.ir/paper/566691/en

    IEEE: Copy

    LIU MIAO, JIN RUN MING, and JIANG YI, “T-BET MEDIATED ANTI-NEOPLASTIC EFFECTS OF DENDRITIC CELL-CYTOKINE INDUCED KILLER CELLS IN VITRO,” IRANIAN JOURNAL OF PEDIATRICS, vol. 22, no. 1, pp. 43–51, 2012, [Online]. Available: https://sid.ir/paper/566691/en

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