PREPARATION AND QUALITY CONTROL OF 177LU-DOTA-ANTI-CD20 FOR RADIO IMMUNOTHERAPY

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YOUSEFNIA HASSAN; JALILIAN AMIR REZA; ZOLGHADRI SAMANEH; SHIRVANI ARANI SIMINDOKHT; BAHRAMI SAMANI ALI; GHANNADI MARAGHEH MOHAMMAD; GHANNADI MARAGHEH MOHAMMAD

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Journal: IRANIAN JOURNAL OF NUCLEAR MEDICINE Year:2010 | Volume:18 | Issue:SUPPLEMENT 1 Page(s): 139-139

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Title

PREPARATION AND QUALITY CONTROL OF 177LU-DOTA-ANTI-CD20 FOR RADIO IMMUNOTHERAPY

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Keywords

LU-177 ANTI-CD20

TARGETTED THERAPY

BIODISTRIBUTION

Abstract

Introduction: The importance of existence and application of radiolabeled anti-CD20 monoclonal antibodies at nonmyeloablative doses in treating B-cell NHL is well recognized throughout the world. In this work, Rituximab was successively labeled with 177Lu-lutetium chloride.Methods: Lu-177 chloride was obtained by thermal neutron flux (4 × 1013 n.cm-2.s-1) of natural Lu2O3 sample with a specific activity of 2.6-3 GBq/mg. The macrocyclic bifunctional chelating agent, Nsuccinimidyl-1, 4, 7, 10-tetraazacyclododecane-1, 4, 7, 10-tetraacetic acid (DOTA-NHS) was prepared followed by conjugation. The radioimmunoconjugate was analyzed for integrity by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Stability of 177Lu-DOTA-rituximab in PBS was determined by storing the final solution at 4°C for 14 days and performing frequent ITLC analysis to determine radiochemical purity followed by administration to wild-type rats for biodistribution studies.Results: DOTA NHS reaction is performed at room temperature and can be done overnight. High uptake in spleen and reticoloendothelial organs was observed. At all time intervals post injection, the activity is mainly removed from the blood which is in agreement with the other reported labeled antibodies. Likewise other radiolabeled proteins the labeled antibody is accumulated in the liver. As a natural reaction to the depletion of the lymphocytes the reticulloendothelial system including spleen will be the final possible reservoir of the depleted lymphocytesConclusion: [177Lu] -DOTA-rituximab is potential radioimmunotherapeutic agent for B-lymphoma treatment.

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APA: Copy

YOUSEFNIA, HASSAN, JALILIAN, AMIR REZA, ZOLGHADRI, SAMANEH, SHIRVANI ARANI, SIMINDOKHT, BAHRAMI SAMANI, ALI, GHANNADI MARAGHEH, MOHAMMAD, & GHANNADI MARAGHEH, MOHAMMAD. (2010). PREPARATION AND QUALITY CONTROL OF ¹⁷⁷LU-DOTA-ANTI-CD20 FOR RADIO IMMUNOTHERAPY. IRANIAN JOURNAL OF NUCLEAR MEDICINE, 18(SUPPLEMENT 1), 139-139. SID. https://sid.ir/paper/571006/en

Vancouver: Copy

YOUSEFNIA HASSAN, JALILIAN AMIR REZA, ZOLGHADRI SAMANEH, SHIRVANI ARANI SIMINDOKHT, BAHRAMI SAMANI ALI, GHANNADI MARAGHEH MOHAMMAD, GHANNADI MARAGHEH MOHAMMAD. PREPARATION AND QUALITY CONTROL OF ¹⁷⁷LU-DOTA-ANTI-CD20 FOR RADIO IMMUNOTHERAPY. IRANIAN JOURNAL OF NUCLEAR MEDICINE[Internet]. 2010;18(SUPPLEMENT 1):139-139. Available from: https://sid.ir/paper/571006/en

IEEE: Copy

HASSAN YOUSEFNIA, AMIR REZA JALILIAN, SAMANEH ZOLGHADRI, SIMINDOKHT SHIRVANI ARANI, ALI BAHRAMI SAMANI, MOHAMMAD GHANNADI MARAGHEH, and MOHAMMAD GHANNADI MARAGHEH, “PREPARATION AND QUALITY CONTROL OF ¹⁷⁷LU-DOTA-ANTI-CD20 FOR RADIO IMMUNOTHERAPY,” IRANIAN JOURNAL OF NUCLEAR MEDICINE, vol. 18, no. SUPPLEMENT 1, pp. 139–139, 2010, [Online]. Available: https://sid.ir/paper/571006/en

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