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Title

SPONTANEOUS EXPRESSION OF NEUROTROPHIC FACTORS AND TH, NURR1, NESTIN GENES IN LONG-TERM CULTURE OF BONE MARROW MESENCHYMAL STEM CELLS

Pages

  243-250

Abstract

 Objective: It has been reported that rat BONE MARROW stromal cells (BMSCs) can be spontaneously differentiated into neural-like cells without any supplemental growth factors and/or chemical treatment after LONG-TERM CULTURE. This study aims to determine Whether, growth factors secreted by MSCs could induce self-differentiation into neural-like cells in a LONG-TERM CULTURE.Materials and Methods: This study consisted of two groups: i. rat BMSCs (passage 5) were cultured in alfa- minimal essential medium (a-MEM) and 10% fetal bovine serum (FBS) without the addition of inducer and exchanging medium for three weeks, as the experimental group and ii. rat BMSCs (passage 5) as the control group. Each group was analysed by reverse transcriptase polymerase chain reaction (RT-PCR) to evaluate the expressions of NEUROTROPHIC FACTORS and neural marker genes.Statistical analyses were carried out using one-way analysis of variance (ANOVA) and Tukey’s multiple comparison with SPSS software (version 16). P<0.05 was considered statistically significant.Results: The experimental group (fifth passage of BMSCs) obtained from adult rats spontaneously differentiated into neural precursor cells after LONG-TERM CULTURE. Cultured cells expressed tyrosine hydroxylase (TH), Nurr1 and nestin genes. Furthermore, some growing cells in suspension became neurosphere-like. Self-differentiated rat MSCs (SDr MSCs) expressed significantly higher levels of NGF (0.96±0.16), nestin (0.63±0.08), and Nurr1 (0.80±0.10) genes (p<0.05).Conclusion: In this study, we reported that rMSCs in LONG-TERM CULTURE underwent spontaneous transformation to neural precursors without the supplement of growth factors and specific chemicals. Cells expressed neural markers such as: TH, Nurr1, and nestin genes.

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    APA: Copy

    MORADI, FATEMEH, HAJI GHASEM KASHANI, MARYAM, GHORBANIAN, MOHAMMAD TAGHI, & LASHKAR BLUKI, TAGHI. (2012). SPONTANEOUS EXPRESSION OF NEUROTROPHIC FACTORS AND TH, NURR1, NESTIN GENES IN LONG-TERM CULTURE OF BONE MARROW MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH), 13(4 (52)), 243-250. SID. https://sid.ir/paper/575708/en

    Vancouver: Copy

    MORADI FATEMEH, HAJI GHASEM KASHANI MARYAM, GHORBANIAN MOHAMMAD TAGHI, LASHKAR BLUKI TAGHI. SPONTANEOUS EXPRESSION OF NEUROTROPHIC FACTORS AND TH, NURR1, NESTIN GENES IN LONG-TERM CULTURE OF BONE MARROW MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2012;13(4 (52)):243-250. Available from: https://sid.ir/paper/575708/en

    IEEE: Copy

    FATEMEH MORADI, MARYAM HAJI GHASEM KASHANI, MOHAMMAD TAGHI GHORBANIAN, and TAGHI LASHKAR BLUKI, “SPONTANEOUS EXPRESSION OF NEUROTROPHIC FACTORS AND TH, NURR1, NESTIN GENES IN LONG-TERM CULTURE OF BONE MARROW MESENCHYMAL STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 13, no. 4 (52), pp. 243–250, 2012, [Online]. Available: https://sid.ir/paper/575708/en

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