DIFFERENT EXPRESSION OF EXTRACELLULAR SIGNAL-REGULATED KINASES (ERK) 1/2 AND PHOSPHO-ERK PROTEINS IN MBA-MB-231 AND MCF-7 CELLS AFTER CHEMOTHERAPY WITH DOXORUBICIN OR DOCETAXEL

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TAHERIAN ALIAKBAR; MAZOOCHI TAHEREH

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Journal: Iranian Journal of Basic Medical Sciences Year:2012 | Volume:15 | Issue:1 Page(s): 669-677

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Title

DIFFERENT EXPRESSION OF EXTRACELLULAR SIGNAL-REGULATED KINASES (ERK) 1/2 AND PHOSPHO-ERK PROTEINS IN MBA-MB-231 AND MCF-7 CELLS AFTER CHEMOTHERAPY WITH DOXORUBICIN OR DOCETAXEL

Author(s)

TAHERIAN ALIAKBAR | MAZOOCHI TAHEREH | Issue Writer Certificate

Keywords

BREAST CANCER

DOCETAXEL

DOXORUBICIN

MCF-7

MDA-MB-231

PHOSPHO-ERK

Abstract

Objective(s): Curative treatment of BREAST CANCER patients using chemotherapy often fails as a result of intrinsic or acquired resistance of the tumor to the drug. ERK is one of the main components of the Ras/Raf/MEK/ERK cascade, which mediates signal from cell surface receptors to transcription factors to regulate different gene expression. In this study, cytotoxicity and the expression of Erk1/2 and PHOSPHO-ERK was compared in MDA-MB-231 (ER-) and MCF-7 (ER+) cell lines after treatment with DOXORUBICIN (DOX) or DOCETAXEL (DOCT).Materials and Methods: Cell cytotoxicity of DOX or DOCT was calculated using MTT assay. Immonofluorescent technique was used to show MDR-1 protein in MDA-MB-231 and MCF-7 cells after treatment with DOX or DOCT. The expression of ERK1/2 and phpspho-ERK was assayed with immunoblotting.Results: Comparing IC50 values showed that MDA-MB-231 cells are more sensitive than MCF-7 cells to DOX or DOCT. Immonofluorescent results confirmed the expression of MDR-1 in these two cell lines after DOX or DOCT treatment. In MDA-MB-231 cells the expression of ERK1/2 and PHOSPHO-ERK was decreased after DOX treatment in a dose-dependent manner. In contrast in MCF-7 cells the expression of ERK1/2 and PHOSPHO-ERK was increased after DOX treatment. DOCT treatment demonstrated the same result with less significant differences than DOX.Conclusion: The heterogeneity seen in cell lines actually reflects the heterogeneity of BREAST CANCERs. That is why, patients categorized in one group respond differently to a single treatment. These results emphasize the importance of a more accurate classification and a more specific treatment of BREAST CANCER subtypes.

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APA: Copy

TAHERIAN, ALIAKBAR, & MAZOOCHI, TAHEREH. (2012). DIFFERENT EXPRESSION OF EXTRACELLULAR SIGNAL-REGULATED KINASES (ERK) 1/2 AND PHOSPHO-ERK PROTEINS IN MBA-MB-231 AND MCF-7 CELLS AFTER CHEMOTHERAPY WITH DOXORUBICIN OR DOCETAXEL. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 15(1), 669-677. SID. https://sid.ir/paper/586520/en

Vancouver: Copy

TAHERIAN ALIAKBAR, MAZOOCHI TAHEREH. DIFFERENT EXPRESSION OF EXTRACELLULAR SIGNAL-REGULATED KINASES (ERK) 1/2 AND PHOSPHO-ERK PROTEINS IN MBA-MB-231 AND MCF-7 CELLS AFTER CHEMOTHERAPY WITH DOXORUBICIN OR DOCETAXEL. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2012;15(1):669-677. Available from: https://sid.ir/paper/586520/en

IEEE: Copy

ALIAKBAR TAHERIAN, and TAHEREH MAZOOCHI, “DIFFERENT EXPRESSION OF EXTRACELLULAR SIGNAL-REGULATED KINASES (ERK) 1/2 AND PHOSPHO-ERK PROTEINS IN MBA-MB-231 AND MCF-7 CELLS AFTER CHEMOTHERAPY WITH DOXORUBICIN OR DOCETAXEL,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 15, no. 1, pp. 669–677, 2012, [Online]. Available: https://sid.ir/paper/586520/en

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