SPECTRAL SEPARATION OF QUANTUM DOTS WITHIN TISSUE EQUIVALENT PHANTOM USING LINEAR UNMIXING METHODS IN MULTISPECTRAL FLUORESCENCE REFLECTANCE IMAGING

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NAJAF ZADE EBRAHIM; HEJAZI MARJANEH; AHMADIAN ALIREZA; MOHAMMADREZA HANIEH

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Journal: Iranian Journal of Medical Physics Year:2012 | Volume:9 | Issue:3 Page(s): 177-182

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Title

SPECTRAL SEPARATION OF QUANTUM DOTS WITHIN TISSUE EQUIVALENT PHANTOM USING LINEAR UNMIXING METHODS IN MULTISPECTRAL FLUORESCENCE REFLECTANCE IMAGING

Author(s)

NAJAF ZADE EBRAHIM | HEJAZI MARJANEH | AHMADIAN ALIREZA | MOHAMMADREZA HANIEH | Issue Writer Certificate

Keywords

FLUORESCENT REFLECTANCE IMAGING

JACOBI SVD

NON-NEGATIVE MATRIX FACTORIZATION

MULTISPECTRAL IMAGING

SVD

Abstract

Introduction: Non-invasive FLUORESCENT REFLECTANCE IMAGING (FRI) is used for accessing physiological and molecular processes in biological media. The aim of this article is to separate the overlapping emission spectra of quantum dots within tissue-equivalent phantom using SVD, SVD/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank"> JACOBI SVD, and NMF methods in the FRI mode.Materials and Methods: In this article, a tissue-like phantom and an optical setup in reflectance mode were developed. The algorithm of MULTISPECTRAL IMAGING method was then written in Matlab environment. The setup included the diode-pumped solid-state lasers at 479 nm, 533 nm, and 798 nm, achromatic telescopic, mirror, high pass and low pass filters, and EMCCD camera. The FRI images were acquired by a CCD camera using band pass filter centered at 600 nm and high pass max at 615 nm for the first region and high pass filter max at 810 nm for the second region. The SVD and SVD/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank"> JACOBI SVD algorithms were written in Matlab environment and compared with a NON-NEGATIVE MATRIX FACTORIZATION (NMF) and applied to the obtained images.Results: PSNR, SNR, CNR of SVD, and NMF methods were obtained as 39 dB, 30.1 dB, and 0.7 dB, respectively. The results showed that the difference of SVD/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank"> JACOBI SVD PSNR with PSNR of NMF and modified NMF algorithm was significant (p<0.0001). The statistical results showed that the SVD/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank"> JACOBI SVD was more accurate than modified NMF.Conclusion: In this study, the SVD/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank"> JACOBI SVD was introduced as a powerful method for obtaining the unmixed FRI images. An experimental evaluation of the algorithm will be done in the near future.

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APA: Copy

NAJAF ZADE, EBRAHIM, HEJAZI, MARJANEH, AHMADIAN, ALIREZA, & MOHAMMADREZA, HANIEH. (2012). SPECTRAL SEPARATION OF QUANTUM DOTS WITHIN TISSUE EQUIVALENT PHANTOM USING LINEAR UNMIXING METHODS IN MULTISPECTRAL FLUORESCENCE REFLECTANCE IMAGING. IRANIAN JOURNAL OF MEDICAL PHYSICS, 9(3), 177-182. SID. https://sid.ir/paper/590041/en

Vancouver: Copy

NAJAF ZADE EBRAHIM, HEJAZI MARJANEH, AHMADIAN ALIREZA, MOHAMMADREZA HANIEH. SPECTRAL SEPARATION OF QUANTUM DOTS WITHIN TISSUE EQUIVALENT PHANTOM USING LINEAR UNMIXING METHODS IN MULTISPECTRAL FLUORESCENCE REFLECTANCE IMAGING. IRANIAN JOURNAL OF MEDICAL PHYSICS[Internet]. 2012;9(3):177-182. Available from: https://sid.ir/paper/590041/en

IEEE: Copy

EBRAHIM NAJAF ZADE, MARJANEH HEJAZI, ALIREZA AHMADIAN, and HANIEH MOHAMMADREZA, “SPECTRAL SEPARATION OF QUANTUM DOTS WITHIN TISSUE EQUIVALENT PHANTOM USING LINEAR UNMIXING METHODS IN MULTISPECTRAL FLUORESCENCE REFLECTANCE IMAGING,” IRANIAN JOURNAL OF MEDICAL PHYSICS, vol. 9, no. 3, pp. 177–182, 2012, [Online]. Available: https://sid.ir/paper/590041/en

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