VALIDATION OF AN IN-HOUSE MADE RAPID UREASE TEST KIT AGAINST THE COMMERCIAL CLO-TEST IN DETECTING HELICOBACTER PYLORI INFECTION IN THE PATIENTS WITH GASTRIC DISORDERS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AMIN SHAHIDI MANELI; FATTAHI MOHAMMAD REZA; FARSHAD SHOHREH; ALBORZI ABDOLVAHAB

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS) Year:2012 | Volume:17 | Issue:3 Page(s): 212-216

Download Full-Text

View:

476

Download:

130

Cites:

Information Journal Paper

Title

VALIDATION OF AN IN-HOUSE MADE RAPID UREASE TEST KIT AGAINST THE COMMERCIAL CLO-TEST IN DETECTING HELICOBACTER PYLORI INFECTION IN THE PATIENTS WITH GASTRIC DISORDERS

Author(s)

AMIN SHAHIDI MANELI | FATTAHI MOHAMMAD REZA | FARSHAD SHOHREH | ALBORZI ABDOLVAHAB | Issue Writer Certificate

Keywords

HELICOBACTER PYLORI

RAPID UREASE TEST

CLO-TEST

GASTRITIS

Abstract

Background: H. pylori is a urease positive organism, and this activity in a gastric biopsy could be considered as a proof of the presence ofH. pylori. For the reasons of high price and difficult accessibility to the commercial CLO-TEST in Iran, we designed an affordable equivalent test with high specificity, accuracy and availability.Methods: Biopsy samples from 80 symptomatic patients with gastrointestinal problems were included in this study. The results of our in-house made rapid urease kit were compared with the commercial CLO-TEST up to 3 hours and 24 hours after inoculation of the biopsy samples. Culture results and gram staining were proposed as gold standard.Results: HELICOBACTER PYLORI was isolated from 36 patients (45.0%) after cultivation of biopsy samples.After 3 hours, 33 (91.6%) cases of positive samples for H. pylori, showed urease positive reaction using both, in-house made and CLO-TEST kits. However, 2 (5.5%) cases showed urease reaction at 24 hours using both the kits. The specificity of 100% was determined for both, in-house made and commercial CLO-TEST kits after 3 hours. The sensitivity for both the kits was estimated at 97.1% after 3hours. However, after 24 hours, sensitivity and specificity of 97.1% and 88.64% was estimated for the in-house and 97.2 % and 95.4% for the commercial CLO-TEST kits, respectively.Conclusion: Specificity and sensitivity of 100% and 97.1 % for up to 3 hours follow biopsy sampling, could be considered as an advantage for our in-house rapid urease kit. Moreover, the rapid urease agar media designed in our lab is cost-effective with adequate sensitivity and specificity levels for the detection of H. pylori, compared with the commercial CLO-TEST.

Cites

GENOTYPING OF THE HELICOBACTER PYLORI CAGA GENE ISOLATED FROM GASTRIC BIOPSIES IN SHIRAZ, SOUTHERN IRAN: A PCR-RFLP AND SEQUENCE ANALYSIS APPROACH

References

No record.

Cite

APA: Copy

AMIN SHAHIDI, MANELI, FATTAHI, MOHAMMAD REZA, FARSHAD, SHOHREH, & ALBORZI, ABDOLVAHAB. (2012). VALIDATION OF AN IN-HOUSE MADE RAPID UREASE TEST KIT AGAINST THE COMMERCIAL CLO-TEST IN DETECTING HELICOBACTER PYLORI INFECTION IN THE PATIENTS WITH GASTRIC DISORDERS. JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS), 17(3), 212-216. SID. https://sid.ir/paper/591330/en

Vancouver: Copy

AMIN SHAHIDI MANELI, FATTAHI MOHAMMAD REZA, FARSHAD SHOHREH, ALBORZI ABDOLVAHAB. VALIDATION OF AN IN-HOUSE MADE RAPID UREASE TEST KIT AGAINST THE COMMERCIAL CLO-TEST IN DETECTING HELICOBACTER PYLORI INFECTION IN THE PATIENTS WITH GASTRIC DISORDERS. JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS)[Internet]. 2012;17(3):212-216. Available from: https://sid.ir/paper/591330/en

IEEE: Copy

MANELI AMIN SHAHIDI, MOHAMMAD REZA FATTAHI, SHOHREH FARSHAD, and ABDOLVAHAB ALBORZI, “VALIDATION OF AN IN-HOUSE MADE RAPID UREASE TEST KIT AGAINST THE COMMERCIAL CLO-TEST IN DETECTING HELICOBACTER PYLORI INFECTION IN THE PATIENTS WITH GASTRIC DISORDERS,” JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS), vol. 17, no. 3, pp. 212–216, 2012, [Online]. Available: https://sid.ir/paper/591330/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops